托瑞米芬对脂代谢的影响与他莫昔芬的体外比较。

The effect of toremifene on lipid metabolism compared with that of tamoxifen in vitro.

机构信息

Department of Breast and Endocrine Surgery, Nagoya University School of Medicine, Nagoya, Japan.

出版信息

Gynecol Obstet Invest. 2011;71(3):213-6. doi: 10.1159/000322372. Epub 2010 Dec 24.

DOI:10.1159/000322372

PMID:21196696

Abstract

BACKGROUND

Tamoxifen (TAM) and toremifene (TOR) prevent estrogen from stimulating breast cancer growth and also have agonistic effects in a number of physiological systems. TAM is known to increase intracellular triglyceride, but the action of TOR on lipid metabolism in vitro has not yet been determined.

AIM

To compare the effect of TOR on lipid metabolism with that of TAM in vitro, using HepG2 cell lines.

METHODS

Intracellular concentrations of total cholesterol and triglyceride in HepG2 cells were measured by an enzymatic method after TAM or TOR treatment.

RESULTS

Intracellular concentrations of total cholesterol were decreased by both TAM and TOR, but not significantly different from the control level. TOR produced no changes in the intracellular concentrations of triglyceride, whereas TAM increased the intracellular concentrations of triglyceride at concentrations ranging from 10(-7) to 10(-5) mol/l of TAM (p < 0.05). Moreover, significant differences were noted between the two treated groups at concentrations ranging from 10(-9) to 10(-5) mol/l (p < 0.05).

CONCLUSION

TOR treatment did not increase intracellular concentrations of triglyceride, although TAM treatment did so.

摘要

背景

他莫昔芬（TAM）和托瑞米芬（TOR）可阻止雌激素刺激乳腺癌生长，并且在许多生理系统中具有激动作用。已知 TAM 可增加细胞内甘油三酯，但 TOR 在体外对脂代谢的作用尚未确定。

目的

用 HepG2 细胞系比较 TOR 与 TAM 在体外对脂代谢的影响。

方法

用酶法测定 TAM 或 TOR 处理后的 HepG2 细胞内总胆固醇和甘油三酯的浓度。

结果

TAM 和 TOR 均可降低细胞内总胆固醇浓度，但与对照组相比无显著差异。TOR 对细胞内甘油三酯浓度无影响，而 TAM 在 10(-7)~~10(-5)mol/L 的浓度范围内增加细胞内甘油三酯浓度（p<0.05）。此外，在 10(-9)~~10(-5)mol/L 的浓度范围内，两个处理组之间存在显著差异（p<0.05）。