实验性糖尿病期间大鼠肝脏D-β-羟丁酸脱氢酶的特定mRNA和多肽含量的变化
Variations of specific mRNA and polypeptide contents of rat liver D-beta-hydroxybutyrate dehydrogenase during an experimental diabetes mellitus.
作者信息
Bailly A, Lone Y C, Latruffe N
机构信息
Laboratoire de Biochimie et Biologie Moléculaire, CNRS UA 531, Université de Franche-Comté, Besançon, France.
出版信息
Biochimie. 1990 May;72(5):351-4. doi: 10.1016/0300-9084(90)90031-b.
The expression of the rat liver D-beta-hydroxybutyrate dehydrogenase (BDH) gene was investigated at different levels: the level of its specific mRNA, the protein content and the enzymatic activity. By using a cDNA probe, we found that the BDH mRNA was about 2 kb and we report here that the decrease of BDH activity in diabetic rats is due to a reduction in the content of the enzyme, which is proportional to a diminution in the amount of the BDH mRNA. We also show that insulin is able to reverse this diabetes effect by restoring the level of BDH mRNA, the BDH content and thus its activity. This result indicates that in vivo the control of the expression of the BDH gene by insulin is mainly transcriptional and/or post-transcriptional (mRNA stability).
我们在不同水平上研究了大鼠肝脏D-β-羟丁酸脱氢酶（BDH）基因的表达：其特异性mRNA水平、蛋白质含量和酶活性。通过使用cDNA探针，我们发现BDH mRNA约为2 kb，并且在此报告糖尿病大鼠中BDH活性的降低是由于该酶含量的减少，这与BDH mRNA量的减少成比例。我们还表明，胰岛素能够通过恢复BDH mRNA水平、BDH含量及其活性来逆转这种糖尿病效应。这一结果表明，在体内胰岛素对BDH基因表达的调控主要是转录水平和/或转录后水平（mRNA稳定性）。
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