三肽和生乳激素对牛乳腺寡肽转运蛋白 2 的影响。
Effects of tripeptides and lactogenic hormones on oligopeptide transporter 2 in bovine mammary gland.
机构信息
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, China.
出版信息
J Anim Physiol Anim Nutr (Berl). 2011 Dec;95(6):781-9. doi: 10.1111/j.1439-0396.2010.01110.x. Epub 2010 Dec 30.
This study was conducted to investigate the expression of oligopeptide transporter 2 (PepT2) and its potential function in bovine mammary gland. First, the PepT2 mRNA and protein were determined in cultured mammary epithelial cells. Then the effects of lactogenic hormones (prolactin, hydrocortisone or insulin) and substrate (threonyl-phenylalanyl-phenylalanine) on PepT2 were investigated. The PepT2 mRNA and protein were successfully detected in bovine mammary epithelial cells. PepT2 gene expression was enhanced by the addition of 50, 500 and 5000 ng/ml prolactin, 10 and 100 ng/ml hydrocortisone, and 50, 500, 5000 and 50,000 ng/ml insulin. PepT2 mRNA abundance was increased when 5, 10 and 15% of threonyl-phenylalanyl-phenylalanine was included. Responses of PepT2 to lactogenic hormones and oligopeptide inferred that it may play an important role in bovine mammary gland.
本研究旨在探讨寡肽转运蛋白 2（PepT2）在牛乳腺中的表达及其潜在功能。首先，在培养的乳腺上皮细胞中测定 PepT2mRNA 和蛋白。然后研究了泌乳激素（催乳素、氢化可的松或胰岛素）和底物（苏氨酰-苯丙氨酰-苯丙氨酸）对 PepT2 的影响。成功检测到牛乳腺上皮细胞中 PepT2mRNA 和蛋白。添加 50、500 和 5000ng/ml 催乳素、10 和 100ng/ml 氢化可的松以及 50、500、5000 和 50000ng/ml 胰岛素均可增强 PepT2 基因表达。当添加 5%、10%和 15%的苏氨酰-苯丙氨酰-苯丙氨酸时，PepT2mRNA 的丰度增加。PepT2 对泌乳激素和寡肽的反应表明，它可能在牛乳腺中发挥重要作用。
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