Immunology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands.
Vaccine. 2011 Feb 4;29(7):1364-73. doi: 10.1016/j.vaccine.2010.12.071. Epub 2011 Jan 1.
Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic intestinal infection of ruminants and has been associated with the etiology of human Crohn's disease. A MAP Hsp70/DDA subunit vaccine previously showed a significant reduction in fecal shedding of MAP in cattle, concomitant with pronounced antibody production against MAP Hsp70, rather than T cell reactivity. Our hypothesis is that if Hsp70-specific antibodies are able to confer protection, the first requisite would be that the Hsp70 molecule is accessible for antibodies in intact MAP bacteria. In the current study monoclonal antibodies identified MAP Hsp70 B cell epitopes. Two linear epitopes were also recognized by antibodies of vaccinated calves and goats. These epitopes showed to be accessible by antibodies in the bacterial cell wall and in intestinal lesional tissue during natural infection. These results indicate that vaccination-induced antibodies can bind intact bacteria and have the potential to contribute to the protective effect of Hsp70/DDA subunit vaccination against bovine paratuberculosis.
分支杆菌副结核亚种（MAP）引起反刍动物的慢性肠道感染，并与人类克罗恩病的病因有关。以前的研究表明，MAP Hsp70/DDA 亚单位疫苗可显著减少牛粪便中 MAP 的脱落，同时产生针对 MAP Hsp70 的明显抗体，而不是 T 细胞反应。我们的假设是，如果 Hsp70 特异性抗体能够提供保护，那么首先要求 Hsp70 分子在完整的 MAP 细菌中能够被抗体识别。在当前的研究中，单克隆抗体鉴定了 MAP Hsp70 B 细胞表位。疫苗接种牛和山羊的抗体还识别了两个线性表位。这些表位显示在天然感染期间细菌细胞壁和肠道病变组织中的抗体可识别。这些结果表明，疫苗诱导的抗体可以结合完整的细菌，并有可能对 Hsp70/DDA 亚单位疫苗接种预防牛副结核病的保护作用做出贡献。
