Department of Neurology The Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Epilepsia. 2011 Jan;52(1):45-52. doi: 10.1111/j.1528-1167.2010.02896.x. Epub 2011 Jan 4.
To analyze activity of identified dentate gyrus granular cells and interneurons during pathologic high-frequency oscillations (pHFOs).
Pilocarpine-treated epileptic mice were anesthetized with urethane and ketamine. Their heads were fixed in a stereotaxic frame. Extracellular unit activity was recoded with glass micropipettes, whereas multiunit and local field activity was simultaneously recorded with attached tungsten microelectrodes. After electrophysiologic experiments, recorded cells were labeled by neurobiotin and visualized by immunohistochemical methods. KEY FINDINGS AND SIGNIFICANCES: pHFOs containing more than three waves were recorded in our experiments, but pathologic single-population spikes also occurred. Identified granular cells discharged preferentially in synchrony with pHFOs and single population spikes, whereas interneurons decreased their discharge frequency during this time. These experiments provide additional confirmation that pHFOs in the dentate gyrus represent single or recurrent population spikes, which in turn reflect summated hypersynchronous discharges of principal cells.
分析病理高频振荡（pHFO）期间被识别的齿状回颗粒细胞和中间神经元的活动。
用尿嘧啶和氯胺酮麻醉匹罗卡品处理的癫痫小鼠。将其头部固定在立体定向框架中。使用玻璃微管记录细胞外单位活动，同时用附着的钨微电极记录多单位和局部场活动。在电生理实验后，用神经生物素标记记录的细胞，并通过免疫组织化学方法进行可视化。
我们的实验记录到了含有三个以上波的 pHFO，但也发生了病理性单峰放电。被识别的颗粒细胞优先与 pHFO 和单峰放电同步放电，而中间神经元在此期间降低了放电频率。这些实验进一步证实，齿状回中的 pHFO 代表单个或复发性群体峰，这反过来反映了主要细胞的总和超同步放电。
