University Josip Juraj Strosmayer Faculty of Medicine Osijek, Osijek, Croatia and Clinical Hospital Center Osijek, Osijek, Croatia University Hospital Zagreb, Zagreb, Croatia.
Scand J Immunol. 2011 Apr;73(4):319-24. doi: 10.1111/j.1365-3083.2010.02506.x.
Cytokine gene polymorphisms have been associated with modified gene expression and cytokine production. Gamma interferon (IFN-γ) plays an important role in the pathogenesis of kidney transplant rejection. This study evaluated the association between IFN-γ gene polymorphisms and the history of acute allograft rejection in 53 adult first-transplant recipients receiving cadaveric kidney grafts. They were followed up in a single centre until 2006, for a median time of 4 years after transplantation (1-22 years). IFN-γ gene polymorphisms +874 T/A (rs2430561) were determined by polymerase chain reaction (PCR). T/T high IFN-γ genotype was found in 12, intermediate T/A in 29 and low A/A in 12 patients. Twenty-six acute kidney rejection episodes were evidenced in 20 patients, of which none occurred in the 12 patients with low IFN-γ genotype A/A. Age, gender, number of HLA (human leukocyte antigen) mismatches, ABO blood groups, HLA, time after transplantation, creatinine clearance and immunosuppressive regimens were excluded as confounding factors associated with IFN-γ genotype distribution between rejectors and non-rejectors. IFN-γ gene polymorphisms could be an important risk factor for acute kidney transplant rejection, whereas the low A/A IFN-γ genotype could be protective against rejection.
细胞因子基因多态性与修饰的基因表达和细胞因子产生有关。γ干扰素（IFN-γ）在肾移植排斥反应的发病机制中起重要作用。本研究评估了 IFN-γ基因多态性与 53 例接受尸体肾移植的成年首次移植受者急性同种异体移植排斥史之间的关系。他们在一个中心进行随访，直到 2006 年，移植后中位数时间为 4 年（1-22 年）。通过聚合酶链反应（PCR）确定 IFN-γ基因多态性+874 T/A（rs2430561）。在 12 例患者中发现 T/T 高 IFN-γ基因型，29 例患者中发现中间 T/A 基因型，12 例患者中发现低 A/A 基因型。在 20 例患者中证实了 26 例急性肾排斥反应发作，其中无一例发生在低 IFN-γ基因型 A/A 的 12 例患者中。年龄、性别、HLA（人类白细胞抗原）错配数量、ABO 血型、HLA、移植后时间、肌酐清除率和免疫抑制方案被排除为与排斥反应和非排斥反应患者 IFN-γ基因型分布相关的混杂因素。IFN-γ基因多态性可能是急性肾移植排斥的重要危险因素，而低 A/A IFN-γ基因型可能对排斥反应具有保护作用。
