利用链霉菌的酪氨酸酶基因探测大肠杆菌的启动子序列。

Use of the tyrosinase gene from Streptomyces to probe promoter sequences for Escherichia coli.

作者信息

Sugiyama M, Nomura H, Nimi O

机构信息

Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.

出版信息

Plasmid. 1990 May;23(3):237-41. doi: 10.1016/0147-619x(90)90055-h.

DOI:10.1016/0147-619x(90)90055-h

PMID:2120717

Abstract

We have constructed a promoter-probe vector utilizing the expression of a promoter-less tyrosinase derived from Streptomyces plasmid pIJ702. The vector, pMX100, has single sites for EcoRI, KpnI, BamHI, XbaI, SalI, and SphI for cloning promoter sequences. When the tac promoter was inserted into pMX100, E. coli harboring the chimeric plasmid produced the melanin pigment.

摘要

我们利用源自链霉菌质粒pIJ702的无启动子酪氨酸酶的表达构建了一个启动子探针载体。该载体pMX100具有EcoRI、KpnI、BamHI、XbaI、SalI和SphI的单一位点，用于克隆启动子序列。当将tac启动子插入pMX100时，携带嵌合质粒的大肠杆菌产生了黑色素。