PHSRN 修饰聚合物对 HUVEC 细胞亲和性评价及整合素介导机制研究。

HUVEC cell affinity evaluation and integrin-mediated mechanism study on PHSRN-modified polymer.

机构信息

Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin, China.

出版信息

Colloids Surf B Biointerfaces. 2011 May 1;84(1):6-12. doi: 10.1016/j.colsurfb.2010.11.032. Epub 2010 Dec 1.

DOI:10.1016/j.colsurfb.2010.11.032

PMID:21216133

Abstract

To investigate the role of the peptide Pro-His-Ser-Arg-Asn (PHSRN) in cell adhesion and growth, PHSRN- and Gly-Arg-Gly-Asp-Ser (GRGDS)-containing polymers (P-PN5 and P-GS5, respectively) were synthesized by modification of poly(D,L-lactide-co-beta-malic acid) (PLMA) with the corresponding peptides. The cell affinities of the modified polymers were evaluated by adhesion and proliferation experiments with human umbilical vein endothelial cells (HUVECs). The results showed that P-PN5 had comparable ability to that of P-GS5 in supporting HUVEC adhesion and growth. Furthermore, the integrin-mediated mechanism of cell-substrate interaction was investigated. The results showed that P-PN5 had similar binding affinity and binding strength towards α(5)β(1) compared to those of P-GS5. The findings suggest that PHSRN is capable of mediating the adhesion and growth of HUVECs independently and that PHSRN-modified polymers might be used as biologically compatible materials.

摘要

为了研究五肽 Pro-His-Ser-Arg-Asn（PHSRN）在细胞黏附和生长中的作用，通过对聚（D，L-乳酸-co-苹果酸）（PLMA）进行相应肽段的修饰，合成了含有 PHSRN 和 Gly-Arg-Gly-Asp-Ser（GRGDS）的聚合物（P-PN5 和 P-GS5）。通过人脐静脉内皮细胞（HUVEC）的黏附和增殖实验评价了修饰聚合物的细胞亲和性。结果表明，P-PN5 具有与 P-GS5 相当的支持 HUVEC 黏附和生长的能力。此外，还研究了整合素介导的细胞-基质相互作用的机制。结果表明，P-PN5 与 P-GS5 相比，对 α(5)β(1)具有相似的结合亲和力和结合强度。这些发现表明 PHSRN 能够独立介导 HUVEC 的黏附和生长，并且 PHSRN 修饰的聚合物可能被用作生物相容材料。