[原始神经外胚层肿瘤中FLI-1的表达及预后因素分析]

[Expression of FLI-1 and analysis of prognostic factors in primitive neuroectodermal tumor].

作者信息

Chen Li-Juan, Jia Yong-Xu, Fan Fei-Fei, Li Xing-Ya

机构信息

Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2010 Dec;32(12):917-20.

PMID:21223800

Abstract

OBJECTIVE

To observe the expression of FLI-1 in primitive neuroectodermal tumors (PNET), explore the value of immunohistochemical staining of FLI-1 in combination with other neural markers in diagnosis of PNET, and analyze the prognostic factors in PNET patients.

METHODS

35 cases of PNET, of which 33 cases with complete clinical data, were included in this study. Immmunohistochemistry (The En Vision method) was applied to detect the expression of FLI-1, CD99, Syn, NSE, S-100, NF, Vim in the tumor tissues. The clinicopathological data of 33 cases were analyzed by Cox regression.

RESULTS

The positive expression rate of FLI-1 were 51.4% and that of CD99 was 88.6%. The sensitivity of FLI-1 combined with CD99 was up to 100%. The positive rates of Vim, Syn, NSE, s-100 and NF were 91.4%, 48.6%, 45.7%, 22.9% and 0, respectively. Cox regression analysis showed that the impact of primary location and treatment modality were of statistical significance (P < 0.05), but the age, sex, stage or size of tumors did not (P > 0.05).

CONCLUSION

Immunohistochemical detection of FLI-1 and neural markers is a preferred method for clinical diagnosis of PNET. The main factors affecting the prognosis are the primary location of PNET and treatment modality.

摘要

目的

观察FLI-1在原始神经外胚层肿瘤（PNET）中的表达，探讨FLI-1免疫组化染色联合其他神经标志物在PNET诊断中的价值，并分析PNET患者的预后因素。

方法

本研究纳入35例PNET患者，其中33例有完整临床资料。采用免疫组织化学（En Vision法）检测肿瘤组织中FLI-1、CD99、Syn、NSE、S-100、NF、Vim的表达。对33例患者的临床病理资料进行Cox回归分析。

结果

FLI-1阳性表达率为51.4%，CD99阳性表达率为88.6%。FLI-1联合CD99的敏感性高达100%。Vim、Syn、NSE、S-100和NF的阳性率分别为91.4%、48.6%、45.7%、22.9%和0。Cox回归分析显示，原发部位和治疗方式的影响具有统计学意义（P<0.05），但年龄、性别、肿瘤分期或大小无统计学意义（P>0.05）。