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蓝舌病病毒血清型1和8疫苗接种对小反刍兽慢病毒感染血清学诊断的干扰。

Interference of vaccination against bluetongue virus serotypes 1 and 8 with serological diagnosis of small-ruminant lentivirus infection.

作者信息

Valas Stephen, Le Ven Alain, Croise Benoit, Maquigneau Maud, Perrin Cécile

机构信息

Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Anses Laboratoire de Niort, B.P. 3081, 60 rue de Pied de Fond, F-79012 Niort Cedex, France.

出版信息

Clin Vaccine Immunol. 2011 Mar;18(3):513-7. doi: 10.1128/CVI.00343-10. Epub 2011 Jan 12.

Abstract

The effects of the recent vaccinations against bluetongue virus serotype 1 (BTV-1) and BTV-8 in Europe on the reliability of enzyme-linked immunosorbent assays (ELISAs) currently used for diagnosis of small-ruminant lentivirus (SRLV) infection were examined. Primary vaccination against BTV-8 in goats induced an increase in reactivity that did not exceed 3 months in a whole-virus indirect ELISA and a competitive ELISA based on the gp135 glycoprotein. Subsequent BTV-1/8 vaccination extended the time scale of false-positive reactivity for up to 6 months. These results are of relevance for SRLV-monitoring programs.

摘要

研究了欧洲近期针对蓝舌病毒血清型1(BTV-1)和BTV-8的疫苗接种对目前用于诊断小反刍兽疫慢病毒(SRLV)感染的酶联免疫吸附测定(ELISA)可靠性的影响。山羊初次接种BTV-8疫苗后,在全病毒间接ELISA和基于gp135糖蛋白的竞争ELISA中,反应性增加,且持续时间不超过3个月。随后接种BTV-1/8疫苗,假阳性反应的时间范围延长至6个月。这些结果与SRLV监测计划相关。

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