Kim J, Foegeding P M
Department of Food Science, North Carolina State University, Raleigh 27695-7624.
J Appl Bacteriol. 1990 Sep;69(3):414-20. doi: 10.1111/j.1365-2672.1990.tb01532.x.
The effects of heat, CaCl2, and ethanol on activation of Bacillus spores were determined by monitoring the absorbance decrease during germination in inosine. Bacillus cereus T, B. subtilis A and B. megaterium QM B1551 spores were activated by heat- and CaCl2-treatments. Ethanol activated B. megaterium and B. subtilis spores yet did not activate B. cereus spores. CaCl2- and ethanol-activations were less effective than heat-activation as judged by optimal germination rates and germination extents. The presence of CaCl2 during heat-treatment inhibited heat-activation of all three Bacillus spores without affecting viability or dipicolinic acid content of the spores. The electrophoretic patterns of coat plus outer membrane proteins extracted from Bacillus spores treated with CaCl2 and heat in the presence of CaCl2 were similar to each other and were distinctively different from the patterns of proteins from unactivated spores or the spores treated with heat and/or ethanol.
通过监测肌苷中芽孢萌发过程中吸光度的降低，测定了热、氯化钙和乙醇对芽孢杆菌芽孢激活的影响。蜡样芽孢杆菌T、枯草芽孢杆菌A和巨大芽孢杆菌QM B1551芽孢通过热处理和氯化钙处理被激活。乙醇可激活巨大芽孢杆菌和枯草芽孢杆菌芽孢，但不能激活蜡样芽孢杆菌芽孢。从最佳萌发率和萌发程度判断，氯化钙和乙醇激活的效果不如热激活。热处理过程中氯化钙的存在抑制了所有三种芽孢杆菌芽孢的热激活，而不影响芽孢的活力或吡啶二羧酸含量。在氯化钙存在的情况下，用氯化钙和热处理的芽孢杆菌芽孢提取的芽孢衣加外膜蛋白的电泳图谱彼此相似，与未激活芽孢或经热和/或乙醇处理的芽孢的蛋白图谱明显不同。
