Institute of Microbiology and Immunology, National Yang-Ming University, Beitou District, Taipei City, Taiwan, ROC.
Antiviral Res. 2011 Mar;89(3):193-8. doi: 10.1016/j.antiviral.2011.01.002. Epub 2011 Jan 15.
Rana catesbeiana ribonuclease (RC-RNase) is a cytotoxic and antitumor RNase isolated from the oocyte yolk granules of the bullfrog R. catesbeiana. Our previous studies have shown that RC-RNase possesses antitumor activity by activating proapoptotic caspases. Here, we demonstrate that RC-RNase also possesses antiviral activity. By using cell viability and caspase activation assays, we show that RC-RNase largely enhances apoptosis of Japanese encephalitis virus (JEV)-infected BHK-21 cells by activating caspase-3, caspase-8, and caspase-9. In addition, immunoblotting experiments revealed that JEV infection enhances the internalization of RC-RNase by cells. In sum, these results indicate that RC-RNase provides a beneficial effect on JEV-infected cells by enhancing apoptosis.
美洲牛蛙核糖核酸酶（RC-RNase）是一种从牛蛙卵黄颗粒中分离得到的细胞毒性和抗肿瘤 RNase。我们之前的研究表明，RC-RNase 通过激活促凋亡半胱天冬酶发挥抗肿瘤活性。在这里，我们证明 RC-RNase 还具有抗病毒活性。通过细胞活力和半胱天冬酶激活测定，我们表明 RC-RNase 通过激活 caspase-3、caspase-8 和 caspase-9 来显著增强乙型脑炎病毒（JEV）感染的 BHK-21 细胞的凋亡。此外，免疫印迹实验表明 JEV 感染增强了细胞对 RC-RNase 的内化。总之，这些结果表明，RC-RNase 通过增强细胞凋亡对 JEV 感染的细胞产生有益的影响。
