Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu 30068, Taiwan.
Neurosci Res. 2011 May;70(1):118-23. doi: 10.1016/j.neures.2011.01.002. Epub 2011 Jan 15.
Dissociated hippocampal neuron culture has long been the model system of choice for many neuroscientists. The ability to culture dissociated hippocampal neurons from genetically modified mice provides an invaluable tool for studying many neuronal processes. In this study, we established a novel method to culture dissociated hippocampal neurons from embryonic and neonatal mice. Dissociated neurons were cultured in a microchamber between the glass coverslip and the plastic cell container without the use of glial feeder cells. Our method significantly simplifies the preparation while produces healthy and long-lived neuronal cultures that are difficult to achieve without the use of feeder cells.
离体海马神经元培养长期以来一直是许多神经科学家的首选模型系统。从基因修饰小鼠中培养离体海马神经元的能力为研究许多神经元过程提供了极其宝贵的工具。在这项研究中，我们建立了一种从胚胎和新生小鼠中培养离体海马神经元的新方法。将分离的神经元在玻璃盖玻片和塑料细胞培养容器之间的微室中培养，而不使用神经胶质饲养细胞。我们的方法大大简化了准备过程，同时产生了健康且寿命长的神经元培养物，如果不使用饲养细胞，很难实现这一点。
