Department of Medicine, School of Health Science, University of A Coruña, Campus de Oza, s/n, 15006, A Coruña, Spain.
Mol Cell Endocrinol. 2011 Mar 30;335(2):177-88. doi: 10.1016/j.mce.2011.01.010. Epub 2011 Jan 15.
Ghrelin, a hormone whose levels increase during food deprivation, plays a pivotal role in the regulation of food intake, energy metabolism and storage, as well as in insulin sensitivity. Here, we investigated the effects of acyl-ghrelin neutralization with the acyl-ghrelin-binding compound NOX-B11(2) during the fasting-refeeding cycle. Our data demonstrate that ghrelin neutralization with NOX-B11(2) impairs recuperation of lost body weight after food deprivation. Analysis of enzymes involved in glucose and lipid metabolism in liver of fed, fasted and refed rats revealed that neutralization of acyl-ghrelin resulted in minor decreases in the enzymes of glycolytic and lipogenic pathways during fasting. However, during refeeding these enzymes as well as glycogen levels recovered more slowly when acyl-ghrelin was blocked. The high levels of ghrelin in response to food deprivation may contribute to an adequate decrease in hepatic glycolytic and lipogenic enzymes and aid in the recovery of body weight and energetic reserves once food becomes available after the fasting period.
胃饥饿素是一种在饥饿时水平升高的激素，在调节食物摄入、能量代谢和储存以及胰岛素敏感性方面发挥着关键作用。在这里，我们研究了在禁食-再喂养周期中用酰基-胃饥饿素结合化合物 NOX-B11(2) 中和酰基-胃饥饿素的效果。我们的数据表明，用 NOX-B11(2) 中和胃饥饿素会损害禁食后体重的恢复。对进食、禁食和再喂食大鼠肝脏中参与葡萄糖和脂质代谢的酶的分析表明，在禁食期间，酰基-胃饥饿素的中和导致糖酵解和生脂途径的酶略有减少。然而，在再喂食期间，当酰基-胃饥饿素被阻断时，这些酶以及糖原水平的恢复更为缓慢。在饥饿时，胃饥饿素水平升高可能有助于适当降低肝脏的糖酵解和生脂酶，并有助于在禁食期后食物供应后恢复体重和能量储备。
