Department of Microbiology, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei, Taiwan.
Curr Opin HIV AIDS. 2011 Jan;6(1):62-7. doi: 10.1097/COH.0b013e3283412568.
To provide updated points of view regarding the recent development and application of in-vitro primary cell models of HIV latency and their potential application in future studies of HIV eradication.
It has been challenging to develop a primary cell model of HIV latency that can authentically recapitulate the quiescent features of resting CD4+ T cells. Recently, several articles have described different approaches that can generate latently HIV-infected resting CD4+ T cells in vitro. Some of them further demonstrated that the primary cell models of HIV latency are suitable for biochemical studies and drug screening.
Recent progress in primary cell models of HIV latency has facilitated research on the mechanisms of HIV latency. These models also serve as a platform for the discovery of drugs that can purge the latent reservoir for HIV in resting CD4+ T cells. These studies will allow deeper insight to the mechanisms governing HIV latency and may help identify some candidate compounds for use in therapeutic strategies for the eradication of latent HIV.
提供关于 HIV 潜伏的体外原代细胞模型的最新进展及其在 HIV 清除的未来研究中的潜在应用的观点。
开发能够真实再现静息 CD4+T 细胞静止特征的 HIV 潜伏原代细胞模型一直具有挑战性。最近，有几篇文章描述了可以在体外产生潜伏感染的静息 CD4+T 细胞的不同方法。其中一些进一步表明，HIV 潜伏的原代细胞模型适合生化研究和药物筛选。
HIV 潜伏的原代细胞模型的最新进展促进了对 HIV 潜伏机制的研究。这些模型也为发现能够清除静息 CD4+T 细胞中潜伏储库的药物提供了一个平台。这些研究将使我们更深入地了解控制 HIV 潜伏的机制，并可能有助于确定一些候选化合物，用于治疗清除潜伏 HIV 的策略。
