Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
Chembiochem. 2011 Jan 24;12(2):224-34. doi: 10.1002/cbic.201000429. Epub 2010 Nov 17.
Mass spectrometry has made many contributions to the chromatin field through the mapping of histone modifications and the identification of protein complexes involved in gene regulation. MS-based proteomics has now evolved from the identification of single protein spots in gels to the identification and quantification of thousands of proteins in complex mixtures. Quantitative approaches also allow comparative and time-resolved analysis of post-translational modifications. An important emerging field is the unbiased interaction analysis of proteins with other proteins, defined protein modifications, specific DNA and RNA sequences, and small molecules. Quantitative proteomics can also accurately monitor whole proteome changes in response to perturbation of the gene expression machinery. We provide an up-to-date review of modern quantitative proteomic technology and its applications in the field of epigenetics.
质谱技术通过对组蛋白修饰的作图以及鉴定参与基因调控的蛋白质复合物，为染色质领域做出了许多贡献。基于 MS 的蛋白质组学已从鉴定凝胶中的单个蛋白质斑点发展到鉴定和定量复杂混合物中的数千种蛋白质。定量方法还允许对翻译后修饰进行比较和时分辨析。一个重要的新兴领域是蛋白质与其他蛋白质、定义的蛋白质修饰、特定的 DNA 和 RNA 序列以及小分子的无偏相互作用分析。定量蛋白质组学还可以准确监测整个蛋白质组对基因表达机制受到干扰时的变化。我们提供了现代定量蛋白质组学技术及其在表观遗传学领域的应用的最新综述。
