Guthrie H D, Bolt D J, Cooper B S
Livest. and Poult. Sci. Inst., Beltsville, Agric. Res. Center, MD 20705.
J Anim Sci. 1990 Nov;68(11):3719-26. doi: 10.2527/1990.68113719x.
This experiment was conducted to compare the ability of USDA porcine FSH-B-1 (pFSH), USDA porcine LH-B-1 (pLH), and pregnant mare's serum gonadotropin (PMSG) to grow large follicles and induce granulosal cell aromatase activity in prepuberal gilts. Twenty-four gilts (164 d old) received one of four treatments by i.m. injection: 1) saline once, n = 8; 2) pFSH (8 micrograms/kg BW, nine times at 8-h intervals), n = 5; 3) pLH (2 micrograms/kg BW, nine times at 8-h intervals), n = 6; or 4) PMSG (15 IU/kg BW, once), n = 5. At slaughter, 72 h after the first injection, the ovaries to saline-treated gilts contained an average of 104 surface antral follicles 1 to 3 mm in diameter. compared to treatment with saline, pFSH increased (P less than .05) the number of follicles 46%, whereas pLH or PMSG decreased (P less than .05) the number by 70 and 84%, respectively. Compared with saline, treatment with PMSG or pLH induced growth of large follicles (7 to 9 mm) (10.8 and 4.8 follicles/gilt, respectively), increased plasma estrogen, increased granulosal cell aromatase activity, and decreased plasma FSH by 51 and 69%; treatment with pFSH had no significant effect on these traits. Results indicate that injected pFSH did not cause growth of large follicles or induce granulosal cell aromatase activity in prepuberal gilts. In contrast, LH initiated growth and increased granulosal cell aromatase activity in a small number of follicles and accelerated atresia among the remaining follicles.
本实验旨在比较美国农业部猪促卵泡素 - B - 1（pFSH）、美国农业部猪促黄体素 - B - 1（pLH）和孕马血清促性腺激素（PMSG）在初情期前小母猪体内促进大卵泡生长及诱导颗粒细胞芳香化酶活性的能力。24头小母猪（164日龄）通过肌肉注射接受四种处理之一：1）生理盐水一次，n = 8；2）pFSH（8微克/千克体重，每8小时一次，共9次），n = 5；3）pLH（2微克/千克体重，每8小时一次，共9次），n = 6；或4）PMSG（15国际单位/千克体重，一次），n = 5。首次注射72小时后屠宰时，生理盐水处理的小母猪卵巢中平均有104个直径1至3毫米的表面有腔卵泡。与生理盐水处理相比，pFSH使卵泡数量增加了46%（P < 0.05），而pLH或PMSG分别使卵泡数量减少了70%和84%（P < 0.05）。与生理盐水相比，PMSG或pLH处理诱导了大卵泡（7至9毫米）的生长（分别为每头小母猪10.8个和4.8个卵泡），增加了血浆雌激素，增加了颗粒细胞芳香化酶活性，并使血浆促卵泡素降低了51%和69%；pFSH处理对这些性状无显著影响。结果表明，注射pFSH不会导致初情期前小母猪体内大卵泡生长或诱导颗粒细胞芳香化酶活性。相反，促黄体素启动了少数卵泡的生长并增加了颗粒细胞芳香化酶活性，同时加速了其余卵泡的闭锁。
