CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, P. R. China.
J Sep Sci. 2011 Jan;34(2):119-26. doi: 10.1002/jssc.201000652. Epub 2010 Dec 9.
An approach was developed for extracting and analyzing the chemical components of tobacco leaves based on solvent extraction and rapid & resolution liquid chromatography/quadrupole time-of-flight mass spectrometry analysis. Two solvents with different polarities were used to extract hydrophilic components and hydrophobic components, respectively, the combined analytical data can provide a "global" view of metabolites. Based on the evaluation of parallel samples, it was found that this approach provided good repeatability, accurate and reliable profiling data, and is suitable for the metabolomics study of tobacco leaves. In order to find the chemical component differences of tobacco leaves, 56 samples from Zimbabwe and China were analyzed using the developed method. The metabolite data were processed by multivariate statistic technique; an obvious group classification between Zimbabwe and China was observed, 14 significantly changed compounds were found, and 9 of them were identified.
建立了一种基于溶剂萃取和快速分辨液相色谱/四极杆飞行时间质谱分析的方法，用于提取和分析烟叶中的化学成分。使用两种不同极性的溶剂分别提取亲水性成分和疏水性成分，组合分析数据可提供代谢物的“全局”视图。基于平行样品的评估，发现该方法提供了良好的重复性、准确可靠的分析数据，适用于烟叶的代谢组学研究。为了找到烟叶化学成分的差异，使用所开发的方法分析了来自津巴布韦和中国的 56 个样本。采用多元统计技术对代谢物数据进行处理；观察到津巴布韦和中国之间存在明显的分组分类，发现 14 种显著变化的化合物，其中 9 种已被鉴定。
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