Department of Chemistry, Burdwan University, Golapbug, Burdwan 713104, West Bengal, India.
Inorg Chem. 2011 Feb 21;50(4):1213-9. doi: 10.1021/ic1015252. Epub 2011 Jan 19.
A new 2,6-bis(5,6-dihydrobenzo[4,5]imidazo[1,2-c]quinazolin-6-yl)-4-methylphenol (1) serves as a highly selective and sensitive fluorescent probe for Zn(2+) in a HEPES buffer (50 mM, DMSO:water = 1:9 (v/v), pH = 7.2) at 25 °C. The increase in fluorescence in the presence of Zn(2+) is accounted for by the formation of dinuclear Zn(2+) complex [Zn(2)(C(35)H(25)N(6)O)(OH)(NO(3))(2)(H(2)O)] (2), characterized by X-ray crystallography. The fluorescence quantum yield of the chemosensor 1 is only 0.019, and it increases more than 12-fold (0.237) in the presence of 2 equiv of the zinc ion. Interestingly, the introduction of other metal ions causes the fluorescence intensity to be either unchanged or weakened. By incubation of cultured living cells (A375 and HT-29) with the chemosensor 1, intracellular Zn(2+) concentrations could be monitored through selective fluorescence chemosensing.
一种新的 2,6-双(5,6-二氢苯并[4,5]咪唑并[1,2-c]喹唑啉-6-基)-4-甲基苯酚（1）在 25°C 的 HEPES 缓冲液（50 mM，DMSO:水=1:9（v/v），pH=7.2）中用作 Zn(2+)的高选择性和高灵敏度荧光探针。在存在 Zn(2+)的情况下荧光强度的增加归因于双核 Zn(2+)配合物[Zn(2)(C(35)H(25)N(6)O)(OH)(NO(3))(2)(H(2)O)]（2）的形成，其结构通过 X 射线晶体学进行了表征。化学传感器 1 的荧光量子产率仅为 0.019，而在存在 2 当量锌离子的情况下增加了 12 倍以上（0.237）。有趣的是，其他金属离子的引入会导致荧光强度不变或减弱。通过将化学传感器 1 与培养的活细胞（A375 和 HT-29）孵育，可以通过选择性荧光化学传感监测细胞内 Zn(2+)浓度。
