Shinozaki Munehisa, Takahashi Yuichiro, Mukaino Masahiko, Saito Nobuhito, Toyama Yoshiaki, Okano Hideyuki, Nakamura Masaya
Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.
J Biomed Biotechnol. 2011;2011:157458. doi: 10.1155/2011/157458. Epub 2010 Dec 28.
In basic research on spinal cord injury (SCI), behavioral evaluation of the SCI animal model is critical. However, it is difficult to accurately evaluate function in the mouse SCI model due to the small size of mice. Although the open-field scoring scale is an outstanding appraisal method, supplementary objective tests are required. Using a compact SCANET system, in which a mouse carries out free movement for 5 min, we developed a novel method to detect locomotor ability. A SCANET system samples the horizontal coordinates of a mouse every 0.1 s, and both the speed and acceleration of its motion are calculated at each moment. It was found that the maximum speed and acceleration of motion over 5 min varied by injury severity. Moreover, these values were significantly correlated with open-field scores. The maximum speed and acceleration of SCI model mice using a SCANET system are objective, easy to obtain, and reproducible for evaluating locomotive function.
在脊髓损伤（SCI）的基础研究中，SCI动物模型的行为评估至关重要。然而，由于小鼠体型小，在小鼠SCI模型中准确评估功能具有一定难度。尽管旷场评分量表是一种出色的评估方法，但仍需要补充客观测试。我们使用一种紧凑的SCANET系统（小鼠在其中自由活动5分钟），开发了一种检测运动能力的新方法。SCANET系统每0.1秒对小鼠的水平坐标进行采样，并计算其在每个时刻的运动速度和加速度。结果发现，5分钟内运动的最大速度和加速度随损伤严重程度而变化。此外，这些值与旷场评分显著相关。使用SCANET系统评估SCI模型小鼠的最大速度和加速度具有客观性、易于获取且可重复，适用于评估运动功能。
