Hasić Sabaheta, Jadrić Radivoj, Kiseljaković Emina, Valjevac Amina, Mornjaković Zakira, Winterhalter-Jadrić Mira
Institute for Physiology and Biochemistry, Medical Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Med Glas (Zenica). 2011 Feb;8(1):140-5.
To develop a rat model of myocardial infarction induced by isoproterenol (ISO). We investigated a type of histological myocardial changes and cardiac troponin I (TnI) kinetic.
The study has used adult, male, Wistar strain rats. Rats were distributed in ISO and control groups. Rats treated with ISO were divided into groups according to the time of cTnI and myocardial lesion analyses: ISO I (30'), ISO II (60'), ISO III (120') and ISO IV (240'). We determined cTnI (Life Diagnostics Inc. West Chester PA, USA) in the serum by ELISA method. We performed histological analysis on the specimens of left ventricular wall stained by hematoxillin-eosin (HE) method.
The first statistically significant rise of cTnI was noted 30 minutes after the ISO administration. There was no statistically significant difference between cTnI mean values among the ISO groups. Observed myocardial histological changes were time dependent.
This model can be suitable for cardioprotective and cardiotoxicity supstance investigations followed by cTnI measurement in blood. The similarity between induced myocardial lesion on animal model in our study and human myocardial lesion in ischemia give us sufficient impulse for further preclinical researches of new cardiac markers.
建立异丙肾上腺素（ISO）诱导的大鼠心肌梗死模型。我们研究了一种组织学心肌变化类型及心肌肌钙蛋白I（TnI）的动力学。
本研究使用成年雄性Wistar品系大鼠。大鼠被分为ISO组和对照组。接受ISO治疗的大鼠根据cTnI和心肌损伤分析时间分为几组：ISO I（30分钟）、ISO II（60分钟）、ISO III（120分钟）和ISO IV（240分钟）。我们采用酶联免疫吸附测定法（ELISA）测定血清中的cTnI（美国宾夕法尼亚州韦斯特切斯特市Life Diagnostics公司）。我们对用苏木精-伊红（HE）染色法染色的左心室壁标本进行组织学分析。
ISO给药后30分钟，首次观察到cTnI有统计学意义的升高。ISO组之间的cTnI均值无统计学意义上的差异。观察到的心肌组织学变化具有时间依赖性。
该模型适用于在测定血液中cTnI之后进行的心脏保护和心脏毒性物质研究。我们研究中动物模型诱导的心肌损伤与人类缺血性心肌损伤之间的相似性为进一步开展新心脏标志物的临床前研究提供了充分的动力。
