Department of Internal Medicine 3 and Institute for Clinical Immunology, University of Erlangen-Nuremberg, Krankenhausstrasse 12, 91054 Erlangen, Germany.
Rheumatology (Oxford). 2011 Oct;50(10):1823-7. doi: 10.1093/rheumatology/keq445. Epub 2011 Jan 25.
To determine the potential of eotaxin-3 as a diagnostic marker for active disease and genetic susceptibility factor for Churg-Strauss syndrome (CSS).
A total of 37 patients with active, relapsed or inactive CSS, 123 healthy controls and 138 disease controls were studied. Clinical data were collected and serum levels of eotaxin-3 were determined. Ex vivo stability of eotaxin-3 in serum samples was tested. Furthermore, the association of single nucleotide polymorphisms (SNPs) in the eotaxin-3 gene with CSS was determined in 161 CSS patients and 124 healthy controls.
Serum eotaxin-3 was highly elevated in active CSS patients. Neither eosinophilic diseases nor other small-vessel vasculitides were associated with high serum eotaxin-3 levels. Receiver operating characteristic curve analysis determined a sensitivity and specificity of 87.5 and 98.6% at a cut-off level of 80 pg/ml. None of the tested SNPs within the eotaxin-3 gene influenced the susceptibility to develop CSS.
Serum eotaxin-3 is a sensitive and specific marker for the diagnosis of active CSS suitable for routine clinical practice. Previously described SNPs in the eotaxin-3 gene do not predict the risk of developing CSS.
确定嗜酸性粒细胞趋化因子 3（eotaxin-3）是否可作为活动性疾病的诊断标志物和变应性肉芽肿性血管炎（CSS）的遗传易感因素。
共研究了 37 例活动性、复发或非活动性 CSS 患者、123 名健康对照者和 138 名疾病对照者。收集临床数据并测定血清 eotaxin-3 水平。检测血清样本中 eotaxin-3 的体外稳定性。此外，在 161 例 CSS 患者和 124 例健康对照者中确定 eotaxin-3 基因中单核苷酸多态性（SNP）与 CSS 的相关性。
活动性 CSS 患者血清 eotaxin-3 水平显著升高。嗜酸性粒细胞疾病或其他小血管血管炎均与高血清 eotaxin-3 水平无关。受试者工作特征曲线分析确定 80 pg/ml 截值时的敏感性和特异性分别为 87.5%和 98.6%。eotaxin-3 基因内未检测到的 SNP 均不影响发生 CSS 的易感性。
血清 eotaxin-3 是诊断活动性 CSS 的敏感和特异标志物，适合常规临床实践。先前描述的 eotaxin-3 基因中的 SNP 不能预测 CSS 的发病风险。
