Fu Rong-guo, Yuan Hao-zheng, Wang Li, Ge Heng, Zhang Jun, Ning Qi-lan, Zhang Fu-jun
Department of Nephrology, Xi an Jiaotong University College of Medicine, Xi an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):96-9.
To investigate the expression of ghrelin and its receptor, growth hormone secretagogue receptor (GHS-R), in the hypothalamus and gastrointestinal tract in rats with chronic renal failure (CRF) and explore their relationship with the disorder of gastrointestinal tract motility.
SD rats were randomly divided into sham-operated group (n=8) and CRF group (n=16), and in the latter group, the rats were subjected to 5/6 nephrectomy to induce CRF. Real-time PCR and immunohistochemical staining were used to detect the distribution of mRNA and protein of ghrelin and GHS-R in the gastric fundus, duodenum, and hypothalamus.
The rats in the CRF group showed a significantly higher expression of ghrelin mRNA and protein in the gastric fundus but a lower expression in the hypothalamus than those in the sham-operated group (P<0.01), but the expression in the duodenum was similar between the two groups (P>0.05). The expression of GHS-R mRNA and protein in the gastric fundus was significantly higher in the CRF group than in the sham-operated group (P<0.01), while in the hypothalamus and duodenum, the expression was significantly lower in the CRF group (P<0.01).
The different distribution patterns of ghrelin and GHS-R in the tissues may be an important pathological basis of gastrointestinal motility disorder in CRF.
探讨慢性肾功能衰竭（CRF）大鼠下丘脑及胃肠道中胃饥饿素及其受体生长激素促分泌素受体（GHS-R）的表达情况，并探讨它们与胃肠道动力障碍的关系。
将SD大鼠随机分为假手术组（n=8）和CRF组（n=16），后者行5/6肾切除术诱导CRF。采用实时荧光定量PCR和免疫组化染色检测胃底、十二指肠及下丘脑中胃饥饿素和GHS-R的mRNA及蛋白分布。
与假手术组相比，CRF组大鼠胃底中胃饥饿素mRNA和蛋白表达显著升高，而下丘脑中表达降低（P<0.01），但两组十二指肠中的表达相似（P>0.05）。CRF组胃底中GHS-R mRNA和蛋白表达显著高于假手术组（P<0.01），而在下丘脑和十二指肠中，CRF组表达显著降低（P<0.01）。
胃饥饿素和GHS-R在组织中的不同分布模式可能是CRF胃肠道动力障碍的重要病理基础。
