对来自墨西哥一个自然保护区的种类繁多的寄生蜂（茧蜂科：矛茧蜂亚科）进行DNA条形码分析。
DNA barcoding a highly diverse group of parasitoid wasps (Braconidae: Doryctinae) from a Mexican nature reserve.
作者信息
Zaldívar-Riverón Alejandro, Martínez Juan José, Ceccarelli Fadia Sara, De Jesús-Bonilla Vladimir Salvador, Rodríguez-Pérez Ana Cecilia, Reséndiz-Flores Andrés, Smith M Alex
机构信息
Colección Nacional de Insectos, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico, D.F, Mexico.
出版信息
Mitochondrial DNA. 2010 Dec;21 Suppl 1:18-23. doi: 10.3109/19401736.2010.523701.
BACKGROUND AND AIMS
The preliminary results of a DNA barcoding study of the doryctine fauna of parasitoid wasps from the Chamela-Cuixmala Biosphere Reserve in Mexico, a region dominated by tropical dry forest, are presented. So far, three field trips have been carried out to the reserve and 468 specimens have been collected, of which 407 cox1 sequences were obtained.
MATERIALS AND METHODS
The general mixed Yule-coalescent model was applied to a phylogram to investigate the number of evolutionary units that can be detected from the DNA sequence data examined.
RESULTS
A total of 185 barcoding species assigned to 20 identified doryctine genera were discriminated using the above model, 115 of which belong to the speciose genus Heterospilus, pointing out the extraordinary species richness of this subfamily of insects in a Mexican tropical dry forest.
CONCLUSION
On the basis of the DNA barcodes generated, Ptesimogastroides Braet & van Achterberg is proposed to be a junior synonym of Ptesimogaster Marsh syn. nov. Neoheterospilus was also found deeply nested within a large Heterospilus clade, suggesting the paraphyly of the latter genus.
背景与目的
本文展示了对来自墨西哥查梅拉-奎克斯马拉生物圈保护区（该地区以热带干旱森林为主）的寄生蜂多里科廷族动物群进行DNA条形码研究的初步结果。到目前为止，已对该保护区进行了三次实地考察，共采集了468个标本，其中获得了407个细胞色素氧化酶亚基1（cox1）序列。
材料与方法
将通用混合尤尔-凝聚模型应用于系统发育树，以研究从所检测的DNA序列数据中可检测到的进化单元数量。
结果
使用上述模型鉴别出了总共185个条形码物种，它们被归入20个已确定的多里科廷属，其中115个属于物种丰富的异距小蜂属，这表明在墨西哥热带干旱森林中，这一昆虫亚科具有非凡的物种丰富度。
结论
基于所生成的DNA条形码，提议将布氏拟腹寄蝇属（Ptesimogastroides Braet & van Achterberg）作为腹寄蝇属（Ptesimogaster Marsh）的次异名（新异名）。还发现新异距小蜂属（Neoheterospilus）深深地嵌套在一个大型异距小蜂属分支内，这表明后者属的并系性。
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