在人类骨肉瘤和软组织肉瘤中，肿瘤抑制因子FUS1蛋白表达常常缺失。
Frequent absence of tumor suppressor FUS1 protein expression in human bone and soft tissue sarcomas.
作者信息
Li Guidong, Kawashima Hiroyuki, Ji Lin, Ogose Akira, Ariizumi Takashi, Umezu Hajime, Xu Yongjun, Hotta Tetsuo, Endo Naoto
机构信息
Division of Orthopedic Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Niigata, Japan.
出版信息
Anticancer Res. 2011 Jan;31(1):11-21.
BACKGROUND
FUS1 is a tumor suppressor gene located on human chromosome 3p21.3. Frequent loss of FUS1 protein expression is associated with lung cancer development. This study examined FUS1 expression and its possible tumor-suppressive role in bone and soft tissue sarcomas.
MATERIALS AND METHODS
The expressions of FUS1 mRNA and FUS1 protein were assessed in sarcoma cell lines, sarcoma tissues, benign bone and soft-tissue tumor (BST) tissues, and healthy tissues. Exogenous FUS1 gene transfection was performed on sarcoma cell lines.
RESULTS
FUS1 mRNA expression was detected in all sarcoma cell lines, all benign BSTs and healthy tissues, and almost all sarcoma tissues. In contrast, FUS1 protein expression was frequently lost in sarcoma cells and sarcoma tissues. The exogenous FUS1 gene delivery induced strong FUS1 protein expression, inhibition of cell viability and apoptosis in sarcoma cells.
CONCLUSION
FUS1 may act as a tumor suppressor in bone and soft-tissue sarcomas.
背景
FUS1是一种位于人类染色体3p21.3上的肿瘤抑制基因。FUS1蛋白表达的频繁缺失与肺癌的发生发展相关。本研究检测了FUS1在骨肉瘤和软组织肉瘤中的表达及其可能的肿瘤抑制作用。
材料与方法
评估FUS1 mRNA和FUS1蛋白在肉瘤细胞系、肉瘤组织、良性骨与软组织肿瘤（BST）组织以及健康组织中的表达。对肉瘤细胞系进行外源性FUS1基因转染。
结果
在所有肉瘤细胞系、所有良性BST和健康组织以及几乎所有肉瘤组织中均检测到FUS1 mRNA表达。相比之下，FUS1蛋白表达在肉瘤细胞和肉瘤组织中经常缺失。外源性FUS1基因传递诱导肉瘤细胞中FUS1蛋白强烈表达、细胞活力抑制和细胞凋亡。
结论
FUS1可能在骨肉瘤和软组织肉瘤中充当肿瘤抑制因子。
Zotero 插件