Department of Chemistry, University of Virginia, Charlottesville, 22904, United States.
Anal Chim Acta. 2011 Feb 21;687(2):150-8. doi: 10.1016/j.aca.2010.12.016. Epub 2010 Dec 16.
Microfluidic technology has been utilized in the development of a modular system for DNA identification through STR (short tandem repeat) analysis, reducing the total analysis time from the ∼6 h required with conventional approaches to less than 3h. Results demonstrate the utilization of microfluidic devices for the purification, amplification, separation and detection of 9 loci associated with a commercially-available miniSTR amplification kit commonly used in the forensic community. First, DNA from buccal swabs purified in a microdevice was proven amplifiable for the 9 miniSTR loci via infrared (IR)-mediated PCR (polymerase chain reaction) on a microdevice. Microchip electrophoresis (ME) was then demonstrated as an effective method for the separation and detection of the chip-purified and chip-amplified DNA with results equivalent to those obtained using conventional separation methods on an ABI 310 Genetic Analyzer. The 3-chip system presented here demonstrates development of a modular, microfluidic system for STR analysis, allowing for user-discretion as to how to proceed after each process during the analysis of forensic casework samples.
微流控技术已被应用于通过 STR（短串联重复序列）分析开发 DNA 鉴定的模块化系统，将总分析时间从传统方法所需的约 6 小时缩短至不到 3 小时。结果表明，微流控设备可用于纯化、扩增、分离和检测与商业上可用于法医界的 miniSTR 扩增试剂盒相关的 9 个位点。首先，通过微设备中的红外（IR）介导的 PCR（聚合酶链反应）证明从口腔拭子中纯化的 DNA 可用于 9 个 miniSTR 基因座的扩增。然后，微芯片电泳（ME）被证明是一种有效的方法，用于分离和检测芯片纯化和芯片扩增的 DNA，其结果与使用 ABI 310 遗传分析仪上的传统分离方法获得的结果相当。本文提出的 3 芯片系统展示了用于 STR 分析的模块化微流控系统的开发，允许用户在法医工作样本分析的每个过程后自行决定如何进行。
