免疫治疗对蜂毒液过敏患者血液树突状细胞的影响。
Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy.
机构信息
Department of Pneumology, University of Rostock, Rostock, Germany.
出版信息
J Allergy Clin Immunol. 2011 Feb;127(2):487-494.e1-3. doi: 10.1016/j.jaci.2010.12.003.
BACKGROUND
Modulation of T-cell differentiation, which is controlled by dendritic cells (DCs), plays a crucial role in specific immunotherapy (SIT). However, the number and the characteristics of blood DCs before and during immunotherapy are unknown.
OBJECTIVE
To analyze the number and the characteristics of blood DC subsets in patients with Hymenoptera venom allergy before and after initiation of SIT.
METHODS
In this clinical trial (NCT00947908), blood myeloid and plasmacytoid DCs were analyzed in 20 patients with Hymenoptera venom allergy (bee or wasp venom) by using 4-color flow cytometry at 3 time points: directly before SIT, and 52 hours and 12 months after initiation of SIT. In addition, 20 age-matched and sex-matched controls were examined.
RESULTS
In patients with Hymenoptera venom allergy, the number of plasmacytoid DCs before SIT was comparable to that of controls. Plasmacytoid DCs decreased markedly 52 hours after initiation of SIT and returned to control levels after 12 months of treatment. Myeloid DCs were elevated in patients with Hymenoptera venom allergy before, during, and after the first 12 months of SIT. In addition, there were changes in the expression of function-associated surface molecules on myeloid DCs (such as Fc γ receptor 2 and Toll-like receptor 2) during SIT.
CONCLUSION
Numbers of blood myeloid DCs are elevated in patients with Hymenoptera venom allergy, and there are specific changes in the expression of function-associated surface molecules on these cells during SIT. Numbers of plasmacytoid DCs in blood are profoundly but are only transiently decreased after initiation of SIT.
背景
树突状细胞（DCs）控制的 T 细胞分化的调节在特异性免疫治疗（SIT）中起着至关重要的作用。然而，在免疫治疗之前和期间血液 DC 的数量和特征尚不清楚。
目的
分析变应原性蜂毒液过敏患者在开始 SIT 前后血液树突状细胞亚群的数量和特征。
方法
在这项临床试验（NCT00947908）中，通过四色流式细胞术分析了 20 例变应原性蜂毒液过敏（蜜蜂或黄蜂毒液）患者（n=20）在 3 个时间点的血液髓样和浆细胞样 DC：在 SIT 之前，SIT 开始后 52 小时和 12 个月。此外，还检查了 20 名年龄和性别匹配的对照者。
结果
在变应原性蜂毒液过敏患者中，SIT 前浆细胞样 DC 的数量与对照组相当。SIT 开始后 52 小时浆细胞样 DC 明显减少，并在治疗 12 个月后恢复到对照水平。在 SIT 的前 12 个月中，变应原性蜂毒液过敏患者的髓样 DC 数量升高。此外，在 SIT 期间髓样 DC 上功能相关表面分子的表达也发生了变化（如 Fcγ受体 2 和 Toll 样受体 2）。
结论
变应原性蜂毒液过敏患者血液中髓样 DC 的数量升高，并且在 SIT 期间这些细胞上功能相关表面分子的表达存在特异性变化。SIT 开始后血液中浆细胞样 DC 的数量明显减少，但只是短暂减少。
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