Ishida M, Utsumi K, Suzaki T, Shigenaka Y
Laboratories of Cell Biology, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
Cell Struct Funct. 1990 Oct;15(5):251-6. doi: 10.1247/csf.15.251.
An electrode sensitive to tetraphenyl phosphonium (TPP+) was applied to monitor the changes in the membrane potential of the ciliate Blepharisma japonicum during the process of cell elongation. Uptake of TPP+ by Blepharisma cells was observed when they were incubated in a medium containing 2 x 10(-6)M TPP+. Subsequent light stimulation (3,000 lux) led to an efflux of accumulated TPP+ from the cells, indicating that depolarization of the plasma membrane occurred with the concomitant cell elongation. TPP+ efflux was always observed whenever cell elongation was induced in response to various stimuli: valinomycin (10(-6)M), K+ (30 mM), Co2+ (5 mM), and Ca2+ (5 mM). Furthermore, elongation was also evoked by electrical stimulation. These observations indicate that membrane depolarization is involved in the process of cell elongation in Blepharisma.
应用对四苯基鏻（TPP+）敏感的电极来监测纤毛虫日本棘尾虫在细胞伸长过程中膜电位的变化。当日本棘尾虫细胞在含有2×10⁻⁶M TPP+的培养基中孵育时，观察到细胞对TPP+的摄取。随后的光刺激（3000勒克斯）导致积累的TPP+从细胞中流出，表明质膜去极化伴随着细胞伸长而发生。每当细胞因各种刺激（缬氨霉素（10⁻⁶M）、K⁺（30 mM）、Co²⁺（5 mM）和Ca²⁺（5 mM））而被诱导伸长时，总是观察到TPP+流出。此外，电刺激也能诱发伸长。这些观察结果表明，膜去极化参与了日本棘尾虫的细胞伸长过程。
