暹罗鳄血液的抗菌和抗真菌活性。

Antibacterial and antifungal activities from Siamese crocodile blood.

作者信息

Leelawongtawon Ratree, Siruntawineti Jindawan, Chaeychomsri Win, Sattaponpan Chisanucha

机构信息

Division of Microbiology and Immunology, Preclinical Science Institute, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.

出版信息

J Med Assoc Thai. 2010 Dec;93 Suppl 7:S58-64.

PMID:21294399

Abstract

OBJECTIVE

To evaluate the in vitro antimicrobial activity of the Siamese crocodile blood against bacteria and fungi.

MATERIAL AND METHOD

Thirty Siamese crocodile blood samples including freeze dried whole blood (FDWB), fresh serum (FS), and freeze dried serum (FDS) were evaluated for antimicrobial susceptibility and MIC values against ATCC-registered strains of nine bacterial species and two fungal species and one fungus isolated from a clinical specimen, by using the standard broth microdilution method and a modified resazurin microtiter plate assay.

RESULTS

The result showed that FS (80 mg/ml) and FDS (100 mg/ml) inhibited Gram negative bacteria including Enterobacter aerogenes ATCC 13048, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 27736, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853 with the susceptibility rate at 23.30%, 10.00%, 40.00%, 70.00%, and 86.67%, respectively for FS, and 30.00%, 10.00%, 43.33%, 76.67% and 90.00%, respectively for FDS. The MIC and MBC were in the range of 12.50-100.00 mg/ml and 25.00-100.00 mg/m1 respectively. FS and FDS also inhibited Cryptococcus neoformans 250309 and Aspergillus niger with the susceptibility rate at 90.00% and 80.00%, respectively for FS and 100.00% and 83.33%, respectively for FDS. The MIC was in the range of 25.00-100.00 mg/ml. However, FS and FDS did not inhibit Gram positive bacteria and did not kill fungi. FDWB (100 mg/ml) could neither inhibit bacteria nor fungi.

CONCLUSION

FS and FDS from Siamese crocodile exhibited potential antibacterial and antifungal activities.

摘要

目的

评估暹罗鳄血液对细菌和真菌的体外抗菌活性。

材料与方法

采用标准肉汤微量稀释法和改良刃天青微量滴定板法，对30份暹罗鳄血液样本进行评估，这些样本包括冻干全血（FDWB）、新鲜血清（FS）和冻干血清（FDS），检测其对9种细菌和2种真菌的美国典型培养物保藏中心（ATCC）注册菌株以及1株从临床标本中分离出的真菌的抗菌敏感性和最低抑菌浓度（MIC）值。

结果

结果显示，FS（80mg/ml）和FDS（100mg/ml）对革兰氏阴性菌有抑制作用，这些革兰氏阴性菌包括产气肠杆菌ATCC 13048、大肠埃希菌ATCC 25922、肺炎克雷伯菌ATCC 27736、鼠伤寒沙门氏菌ATCC 13311和铜绿假单胞菌ATCC 27853，FS的敏感率分别为23.30%、10.00%、40.00%、70.00%和86.67%，FDS的敏感率分别为30.00%、10.00%、43.33%、76.67%和90.00%。MIC和最低杀菌浓度（MBC）分别在12.50 - 100.00mg/ml和25.00 - 100.00mg/ml范围内。FS和FDS对新型隐球菌250309和黑曲霉也有抑制作用，FS的敏感率分别为90.00%和80.00%，FDS的敏感率分别为100.00%和83.33%。MIC在25.00 - 100.00mg/ml范围内。然而，FS和FDS对革兰氏阳性菌无抑制作用，对真菌也无杀灭作用。FDWB（100mg/ml）既不能抑制细菌也不能抑制真菌。