Saekoo Jiraporn, Dechsukum Chavaboon, Graidist Potchanapond, Itharat Arunporn
Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
J Med Assoc Thai. 2010 Dec;93 Suppl 7:S277-82.
Dioscorealide B is an active compound from the rhizome of Dioscorea membranacea Pierre which locally known as "Hua-Khao-Yen". This medicinal plant has long been used in the anticancer prescription of Thai traditional medicine.
To examine the cytotoxic effect and mechanism of Dioscorealide B in human breast carcinoma cells.
Dioscorealide B was isolated from the rhizome of Hua KhaoYen (Dioscorea membranacea).The cytotoxicity of Dioscorealide B was evaluated in two human breast cancer cell lines, MCF-7 and MDA-MB 468 by Sulphorhodamine B (SRB) assay. RT-PCR and Caspase-Glo assay were used to further elucidate its cytotoxic mechanism.
Dioscorealide B showed cytotoxic effect on MCF-7 (IC50 = 2.76 microM) and MDA-MB 468 (IC50 = 9.93 microM). The mRNA level for p53, p21 and Bax were increased while Bcl-2 was decreased after the treatment. MCF-7 treated with Dioscorealide B showed the induction of apoptosis via the activation of caspase-9 and -7.
The results suggested that the mechanisms of Dioscorealide B might be involved in p53 and the intrinsic apoptotic pathway.
薯蓣内酯B是从当地称为“Hua-Khao-Yen”的薯蓣科植物（Dioscorea membranacea Pierre）根茎中提取的一种活性化合物。这种药用植物长期以来一直用于泰国传统医学的抗癌处方中。
研究薯蓣内酯B对人乳腺癌细胞的细胞毒性作用及其机制。
从Hua KhaoYen（Dioscorea membranacea）根茎中分离出薯蓣内酯B。采用磺酰罗丹明B（SRB）法评估薯蓣内酯B对两种人乳腺癌细胞系MCF-7和MDA-MB 468的细胞毒性。采用逆转录聚合酶链反应（RT-PCR）和Caspase-Glo检测法进一步阐明其细胞毒性机制。
薯蓣内酯B对MCF-7（IC50 = 2.76 microM）和MDA-MB 468（IC50 = 9.93 microM）显示出细胞毒性作用。处理后，p53、p21和Bax的mRNA水平升高，而Bcl-2水平降低。用薯蓣内酯B处理的MCF-7细胞通过激活caspase-9和-7诱导凋亡。
结果表明，薯蓣内酯B的作用机制可能与p53及内源性凋亡途径有关。
