绵萆薢中薯蓣皂苷元B对乳腺癌细胞的细胞毒性作用及其机制
Cytotoxic effect and its mechanism of dioscorealide B from Dioscorea membranacea against breast cancer cells.
作者信息
Saekoo Jiraporn, Dechsukum Chavaboon, Graidist Potchanapond, Itharat Arunporn
机构信息
Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
出版信息
J Med Assoc Thai. 2010 Dec;93 Suppl 7:S277-82.
BACKGROUND
Dioscorealide B is an active compound from the rhizome of Dioscorea membranacea Pierre which locally known as "Hua-Khao-Yen". This medicinal plant has long been used in the anticancer prescription of Thai traditional medicine.
OBJECTIVE
To examine the cytotoxic effect and mechanism of Dioscorealide B in human breast carcinoma cells.
MATERIAL AND METHOD
Dioscorealide B was isolated from the rhizome of Hua KhaoYen (Dioscorea membranacea).The cytotoxicity of Dioscorealide B was evaluated in two human breast cancer cell lines, MCF-7 and MDA-MB 468 by Sulphorhodamine B (SRB) assay. RT-PCR and Caspase-Glo assay were used to further elucidate its cytotoxic mechanism.
RESULTS
Dioscorealide B showed cytotoxic effect on MCF-7 (IC50 = 2.76 microM) and MDA-MB 468 (IC50 = 9.93 microM). The mRNA level for p53, p21 and Bax were increased while Bcl-2 was decreased after the treatment. MCF-7 treated with Dioscorealide B showed the induction of apoptosis via the activation of caspase-9 and -7.
CONCLUSION
The results suggested that the mechanisms of Dioscorealide B might be involved in p53 and the intrinsic apoptotic pathway.
背景
薯蓣内酯B是从当地称为“Hua-Khao-Yen”的薯蓣科植物（Dioscorea membranacea Pierre）根茎中提取的一种活性化合物。这种药用植物长期以来一直用于泰国传统医学的抗癌处方中。
目的
研究薯蓣内酯B对人乳腺癌细胞的细胞毒性作用及其机制。
材料与方法
从Hua KhaoYen（Dioscorea membranacea）根茎中分离出薯蓣内酯B。采用磺酰罗丹明B（SRB）法评估薯蓣内酯B对两种人乳腺癌细胞系MCF-7和MDA-MB 468的细胞毒性。采用逆转录聚合酶链反应（RT-PCR）和Caspase-Glo检测法进一步阐明其细胞毒性机制。
结果
薯蓣内酯B对MCF-7（IC50 = 2.76 microM）和MDA-MB 468（IC50 = 9.93 microM）显示出细胞毒性作用。处理后，p53、p21和Bax的mRNA水平升高，而Bcl-2水平降低。用薯蓣内酯B处理的MCF-7细胞通过激活caspase-9和-7诱导凋亡。
结论
结果表明，薯蓣内酯B的作用机制可能与p53及内源性凋亡途径有关。
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