抗组织型纤溶酶原激活剂单克隆抗体的制备与鉴定
Production and characterization of monoclonal antibodies against tissue-type plasminogen activator.
作者信息
Ding H, Wang J Y, Song H Y
机构信息
Department of Molecular Genetics, Shanghai Medical University.
出版信息
Chin J Biotechnol. 1990;6(3):223-7.
Four hybridoma clones, TA1, TA2, TA3 and TA4, producing monoclonal antibodies against t-PA were obtained by fusion of mouse myeloma cells (SP2/0 or NS-1) with mouse spleen cells previously immunized with purified t-PA. The antibody titers of the four hybridoma ascites were higher than 1 x 10(5) determined by ELISA. The double immune diffusion test showed that all hybridoma supernatants contained mouse IgG1. These monoclonal antibodies reacted only with t-PA, and rt-PA prepared by genetic engineering, but not with UK. t-PA, activity was inhibited by these monoclonal antibodies completely.
通过将小鼠骨髓瘤细胞（SP2/0或NS-1）与先前用纯化的组织型纤溶酶原激活剂（t-PA）免疫的小鼠脾细胞融合，获得了四个产生抗t-PA单克隆抗体的杂交瘤克隆TA1、TA2、TA3和TA4。通过酶联免疫吸附测定（ELISA）确定，这四种杂交瘤腹水的抗体效价均高于1×10⁵。双向免疫扩散试验表明，所有杂交瘤上清液均含有小鼠IgG1。这些单克隆抗体仅与t-PA以及通过基因工程制备的重组t-PA（rt-PA）发生反应，而不与尿激酶（UK）反应。这些单克隆抗体可完全抑制t-PA的活性。
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