Zhang Da-wu, Zhang Lei, Liu Jian-gang
Department of Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Dec;30(12):1279-83.
To investigate effect of Xiongshao Capsule (XSC) combined with ischemic postconditioning (IPoC) on tumor necrosis factor-alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1) contents as well as inflammatory cell infiltration (ICI) in myocardium of rat with ischemic reperfusion (I/R) injury.
Seventy-five Sprague-Dawley rats were equally randomized into 5 groups, the sham-operated group (A), the I/R group (B), the IPoC group (C), the fosinopril sodium plus IPoC group (D), and the XSC plus IPoC group (E). Excepting rats in Group A, all animals received I/R injury through a 30-min occlusion of left anterior descending artery followed by 1-h reperfusion. Additionally, IPoC (3 cycles of 10 s reperfusion/10 s of ischemia) was applied on rats in Group C before 1 h of reperfusion; while rats in Groups D and E were pretreated for 14 days with 0.9 mg/kg fosinopril sodium and 0.135 g/kg XSC respectively via gastrogavage, and the I/R injury with IPoC applied 2 h after the final gavage. Serum creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT) levels were detected by colorimetric method, myocardial infarction size was measured by nitro blue tetrazolium chloride (NBT) staining, MCP-1 and TNF-alpha contents in myocardial tissue were examined by enzyme-linked immunosorbent assay (ELISA), and ICI was detected by HE staining.
Compared with Group B, myocardial enzymes and infarction size were significantly decreased (P<0.01), contents of MCP-1, TNF-alpha and ICI in myocardial tissue were significantly decreased (P<0.05, P<0.01) in Group C. Compared with Group C, further reduced infarction size and release of myocardial enzyme CK-MB (P<0.01) were seen in Group E, and contents of MCP-1 and TNF-alpha as well as ICI in myocardial tissue in Group E were also significantly lower (P<0.05, P<0.01).
XSC could enhance the protective effect of IPoC on rat with myocardial I/R injury, and the mechanism may be related to its inhibition on MCP-1 and TNF-alpha expressions as well as ICI suppression.
探讨芎芍胶囊（XSC）联合缺血后处理（IPoC）对缺血再灌注（I/R）损伤大鼠心肌中肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白-1（MCP-1）含量及炎性细胞浸润（ICI）的影响。
将75只Sprague-Dawley大鼠随机均分为5组，即假手术组（A组）、I/R组（B组）、IPoC组（C组）、福辛普利钠+IPoC组（D组）和XSC+IPoC组（E组）。除A组大鼠外，其余动物均通过结扎左冠状动脉前降支30分钟后再灌注1小时建立I/R损伤模型。此外，C组大鼠在再灌注前1小时进行IPoC（10秒再灌注/10秒缺血，共3个周期）；D组和E组大鼠分别通过灌胃给予0.9mg/kg福辛普利钠和0.135g/kg XSC预处理14天，末次灌胃2小时后进行I/R损伤并给予IPoC。采用比色法检测血清肌酸激酶同工酶（CK-MB）和心肌肌钙蛋白T（cTnT）水平，用氯化硝基四氮唑蓝（NBT）染色法测量心肌梗死面积，采用酶联免疫吸附测定（ELISA）法检测心肌组织中MCP-1和TNF-α含量，用苏木精-伊红（HE）染色法检测ICI。
与B组相比，C组心肌酶水平和梗死面积显著降低（P<0.01），心肌组织中MCP-1、TNF-α含量及ICI显著减少（P<0.05，P<0.01）。与C组相比，E组梗死面积进一步减小，心肌酶CK-MB释放进一步减少（P<0.01），心肌组织中MCP-1、TNF-α含量及ICI也显著降低（P<0.05，P<0.01）。
芎芍胶囊可增强IPoC对大鼠心肌I/R损伤的保护作用，其机制可能与抑制MCP-1和TNF-α表达及减轻ICI有关。
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