嗜盐菌视紫红质在温和非离子去污剂正辛基-β-硫代葡萄糖苷作用下的增溶及结构稳定性

Solubilization and structural stability of bacteriorhodopsin with a mild nonionic detergent, n-Octyl-β-thioglucoside.

作者信息

Asada Azusa, Sonoyama Masashi

机构信息

Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Gunma, Japan.

出版信息

Biosci Biotechnol Biochem. 2011;75(2):376-8. doi: 10.1271/bbb.100726. Epub 2011 Feb 7.

DOI:10.1271/bbb.100726

PMID:21307577

Abstract

Solubilization and structural stability of a membrane protein bacteriorhodopsin (bR) with n-octyl-β-thioglucoside (OTG) was investigated in comparison with a previous study on bR solubilized with n-octyl-β-glucoside (OG). Highly efficient and stable solubilization of bR with OTG was accomplished above the OTG concentration of about 15 mM. In comparison with OG-solubilized bR, the structural stability of OTG-solubilized bR was high in the dark and under light illumination. These results indicate that OTG is a detergent superior to OG for solubilizing bR molecules.

摘要

与之前关于用正辛基-β-D-葡萄糖苷（OG）溶解细菌视紫红质（bR）的研究相比，研究了用正辛基-β-硫代葡萄糖苷（OTG）对膜蛋白细菌视紫红质（bR）的增溶作用和结构稳定性。在OTG浓度约为15 mM以上时，实现了bR的高效稳定增溶。与OG溶解的bR相比，OTG溶解的bR在黑暗和光照条件下结构稳定性较高。这些结果表明，在溶解bR分子方面，OTG是一种优于OG的去污剂。