Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(3):281-90. doi: 10.1080/10934529.2011.535435.
Bacterial diversity dynamics were investigated in the soil samples in different distances and depths from/at a long-term petroleum-contaminated site. Microbial activity in the soil samples showed ATP values closely correlated with organic matter content (OC) and total petroleum hydrocarbon (TPH). Bacterial community diversity (H) and evenness (J) using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and PCR-T-RFLP (terminal restriction fragment length polymorphism) results showed positive correlation with concentration of TPH or OC, but tmoA (toluene monooxygenase gene)-based bacterial H and J using a PCR-T-RFLP result did not. No significant difference of H and J values in the bacterial and the tmoA communities was observed. The bacterial community structure characterized by PCR-DGGE and PCR-T-RFLP techniques showed similarity according to soil sampling distance rather than soil sampling depth. Canonical correspondence analysis demonstrated that OC including TPH had the most significant effect on the bacterial community diversity at the long-term petroleum-contaminated site.
对长期受石油污染场地不同距离和深度的土壤样本中的细菌多样性动态进行了研究。土壤样本中的微生物活性表现出 ATP 值与有机质含量（OC）和总石油烃（TPH）密切相关。使用 PCR-DGGE（聚合酶链反应-变性梯度凝胶电泳）和 PCR-T-RFLP（末端限制性片段长度多态性）技术的细菌群落多样性（H）和均匀度（J）结果显示与 TPH 或 OC 浓度呈正相关，但基于 tmoA（甲苯单加氧酶基因）的细菌 H 和 J 的 PCR-T-RFLP 结果并非如此。在细菌和 tmoA 群落中，H 和 J 值没有明显差异。根据土壤采样距离而不是土壤采样深度，PCR-DGGE 和 PCR-T-RFLP 技术表征的细菌群落结构具有相似性。典范对应分析表明，OC 包括 TPH 对长期受石油污染场地的细菌群落多样性有最显著的影响。
