Department of Oral Microbiology, Research Institute MOVE, University of Amsterdam, TheNetherlands.
J Periodontal Res. 2011 Aug;46(4):407-16. doi: 10.1111/j.1600-0765.2011.01353.x. Epub 2011 Feb 17.
Inflammatory responses of host cells to oral pathogenic bacteria, such as Porphyromonas gingivalis, are crucial in the development of periodontitis. Host cells, such as periodontal ligament and gingival fibroblasts, from periodontitis patients may respond to P. gingivalis in a different manner compared with cells from healthy persons. The aim of this study was to investigate inflammatory responses to viable P. gingivalis by periodontal ligament and gingival fibroblasts from periodontitis patients and healthy control subjects.
Primary periodontal ligament and gingival fibroblasts from periodontitis patients (n=14) and healthy control subjects (n=8) were challenged in vitro with viable P. gingivalis. Gene expression of Toll-like receptors (TLRs) 1, 2, 4, 6, 7 and 9, CD14, nuclear factor-κB1 and its putative inhibitor NF-κB inhibitor-like protein1, and of interleukin-1β, interleukin-6, interleukin-8, tumour necrosis factor-α, monocyte chemotactic protein-1 and regulated upon activation, normal T-cel expressed, and secreted, were assessed by real-time PCR.
Periodontal ligament fibroblasts from periodontitis patients had a higher mRNA expression of TLR1, TLR4, TLR7 and CD14, and a lower expression of NFKBIL1, both before and after P. gingivalis challenge. In contrast, gingival fibroblasts from periodontitis patients had stronger induction of TLR1, TLR2 and TLR7 by P. gingivalis. Cytokine responses were not different between patients and control subjects. Interestingly, periodontal ligament, but not gingival, fibroblasts from P. gingivalis culture-positive persons responded more strongly to P. gingivalis than periodontal ligament fibroblasts from P. gingivalis-negative persons.
Periodontal ligament and gingival fibroblasts respond to P. gingivalis in a different manner and may play different roles in periodontitis. Both subsets of fibroblasts from patients appear more active in interaction with P. gingivalis. Moreover, periodontal ligament fibroblasts from P. gingivalis-positive donors are more responsive to an in vitro P. gingivalis challenge.
宿主细胞对口腔致病菌（如牙龈卟啉单胞菌）的炎症反应在牙周炎的发生发展中起着至关重要的作用。与健康个体的细胞相比，牙周炎患者的牙周韧带和牙龈成纤维细胞可能对牙龈卟啉单胞菌的反应有所不同。本研究旨在探讨牙周炎患者和健康对照者牙周韧带和牙龈成纤维细胞对活牙龈卟啉单胞菌的炎症反应。
体外培养牙周炎患者（n=14）和健康对照者（n=8）的原代牙周韧带和牙龈成纤维细胞，用活牙龈卟啉单胞菌刺激。采用实时 PCR 检测 Toll 样受体（TLR）1、2、4、6、7 和 9、CD14、核因子-κB1 及其假定抑制剂 NF-κB 抑制剂样蛋白 1 以及白细胞介素-1β、白细胞介素-6、白细胞介素-8、肿瘤坏死因子-α、单核细胞趋化蛋白-1 和活化后正常 T 细胞表达和分泌的基因表达。
牙周炎患者的牙周韧带成纤维细胞在牙龈卟啉单胞菌刺激前后 TLR1、TLR4、TLR7 和 CD14 的 mRNA 表达较高，而 NFKBIL1 的表达较低。相比之下，牙周炎患者的牙龈成纤维细胞对牙龈卟啉单胞菌的 TLR1、TLR2 和 TLR7 诱导作用更强。患者和对照组之间的细胞因子反应没有差异。有趣的是，与牙龈卟啉单胞菌阴性者相比，牙周韧带而非牙龈成纤维细胞对牙龈卟啉单胞菌培养阳性者的反应更为强烈。
牙周韧带和牙龈成纤维细胞对牙龈卟啉单胞菌的反应方式不同，在牙周炎中可能发挥不同的作用。与健康个体相比，患者来源的两种成纤维细胞亚群与牙龈卟啉单胞菌相互作用时更为活跃。此外，牙龈卟啉单胞菌阳性供体的牙周韧带成纤维细胞对体外牙龈卟啉单胞菌刺激的反应更为敏感。
