Key Laboratory of Pollution Process and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Chemosphere. 2011 May;83(8):1080-6. doi: 10.1016/j.chemosphere.2011.01.046. Epub 2011 Feb 18.
Although polycyclic musks have been shown to cause lethal and sub-lethal effects on organisms, their biochemical toxicity to earthworms is not well understood. In the current study, we investigated the responses of antioxidant systems and lipid peroxidation after exposing Eisenia fetida to soil contaminated with 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl-cyclopenta-γ-2-benzopyran (HHCB). Significant increase in lipid peroxidation level was observed on day 14 at two high concentrations, 50 and 100 mg kg(-1). Among antioxidant enzymes, the primary response to chronic HHCB exposure can be attributed to superoxide dismutase (SOD) and catalase (CAT). Of the two enzymes, SOD exhibited more sensitive response to HHCB stress. In addition, these two enzymes could have a combined effect on fighting damage by reactive oxygen species, evidenced by a marked relationship between lipid peroxidation and enzyme activity. On the other hand, dose-dependent inhibition of peroxidase (POD) activity has been observed throughout the test. The results suggest that the variations in investigated parameters of E. fetida could be used as responsive biomarkers for oxidative stress caused by HHCB in a soil environment.
尽管多环麝香已被证明对生物具有致命和亚致死影响，但它们对蚯蚓的生化毒性还不太清楚。在本研究中，我们研究了暴露于受 1,3,4,6,7,8-六氢-4,6,6,7,8,8-六甲基-环戊基-γ-2-苯并吡喃（HHCB）污染土壤后，抗氧化系统和脂质过氧化的反应。在两个高浓度（50 和 100 mg kg(-1)）下，在第 14 天观察到脂质过氧化水平显著增加。在抗氧化酶中，对慢性 HHCB 暴露的主要反应可归因于超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。在这两种酶中，SOD 对 HHCB 胁迫的反应更为敏感。此外，这两种酶可能对活性氧引起的损伤具有协同作用，这可以通过脂质过氧化和酶活性之间的显著关系来证明。另一方面，在整个试验过程中，过氧化物酶（POD）活性呈剂量依赖性抑制。结果表明，E. fetida 研究参数的变化可用作 HHCB 在土壤环境中引起氧化应激的反应性生物标志物。
