Department of Chemistry, University of Quebec in Montreal, C.P. 8888, Succ. Centre-Ville, Montreal, Quebec, Canada H3C 3P8.
Ecotoxicol Environ Saf. 2011 May;74(4):1021-6. doi: 10.1016/j.ecoenv.2011.01.016. Epub 2011 Feb 19.
Contamination of water bodies by saxitoxin can result in various toxic effects in aquatic organisms. Saxitoxin contamination has also been shown to be a threat to human health in several reported cases, even resulting in death. In this study, we evaluated the sensitivity of animal (Neuro-2A) and algal (Chlamydomonas reinhardtii) bioassays to saxitoxin effect. Neuro-2A cells were found to be sensitive to saxitoxin, as shown by a 24 h EC50 value of 1.5 nM, which was obtained using a cell viability assay. Conversely, no saxitoxin effect was found in any of the algal biomarkers evaluated, for the concentration range tested (2-128 nM). These results indicate that saxitoxin may induce toxic effects in animal and human populations at concentrations where phytoplankton communities are not affected. Therefore, when evaluating STX risk of toxicity, algal bioassays do not appear to be reliable indicators and should always be conducted in combination with animal bioassays.
水体受到石房蛤毒素污染会对水生生物产生各种毒性影响。有几例报告显示，石房蛤毒素污染也会威胁到人类健康，甚至导致死亡。在这项研究中，我们评估了动物（Neuro-2A）和藻类（莱茵衣藻）生物测定法对石房蛤毒素效应的敏感性。神经细胞系（Neuro-2A）对石房蛤毒素敏感，细胞活力测定法得到的 24 h EC50 值为 1.5 nM。相反，在所测试的浓度范围内（2-128 nM），没有发现藻类生物标志物受到石房蛤毒素的影响。这些结果表明，在浮游植物群落不受影响的浓度下，石房蛤毒素可能会对动物和人类群体产生毒性影响。因此，在评估石房蛤毒素毒性风险时，藻类生物测定法似乎不是可靠的指标，应始终与动物生物测定法结合使用。
