Wang Xin-Xing, Li Jing-Xiao, Wang Wen-Guang, Sun Xiao-Mei, He Chun-Yan, Dai Jie-Jie
Institute of Medical Biology, Chinese Academy of Medical Sciences/Peking Union Medical College, Kunming Yunnan 650118, China.
Dongwuxue Yanjiu. 2011 Feb;32(1):66-9. doi: 10.3724/SP.J.1141.2011.01066.
Virological testing and monitoring is a fundamental part of quality control of experimental animals. However, there are few papers regarding the spectrum and status of natural infection in wild tree shrews with human and animal pathogenic viruses. Using enzyme-linked immunosorbent adsorption assay (ELISA), we tested sixty wild tree shrews captured from Qinglong, an outskirt region of Kunming, Yunnan Province, China for eleven viruses, including herpes simplex virus, coxsackie virus, influenza virus, HAV, HBV, HCV, HDV, dengue virus, hemorrhagic fever virus and measles virus. Our results showed that, in the serum samples, 22/60 (36.7%) and 1/60 (1.67%) were antibody positive for herpes simplex virus and coxsackie virus, respectively, and 4/60 (6.7%) were antigen positive for rotavirus in the feces. The remaining species of viruses were negative in these tree shrews. Based on these results, we propose that herpes simplex virus, coxsackie virus and cotavirus should be listed as top priority for routine virological monitoring of tree shrews.
病毒学检测与监测是实验动物质量控制的基本组成部分。然而，关于野生树鼩感染人类和动物致病病毒的自然感染谱及状况的论文较少。我们采用酶联免疫吸附测定（ELISA）法，对从中国云南省昆明市郊区青龙捕获的60只野生树鼩进行了11种病毒的检测，这些病毒包括单纯疱疹病毒、柯萨奇病毒、流感病毒、甲型肝炎病毒、乙型肝炎病毒、丙型肝炎病毒、丁型肝炎病毒、登革热病毒、出血热病毒和麻疹病毒。我们的结果显示，血清样本中，单纯疱疹病毒和柯萨奇病毒的抗体阳性率分别为22/60（36.7%）和1/60（1.67%），粪便中轮状病毒的抗原阳性率为4/60（6.7%）。其余病毒在这些树鼩中均为阴性。基于这些结果，我们建议单纯疱疹病毒、柯萨奇病毒和轮状病毒应列为树鼩常规病毒学监测的首要重点。
