Fernández-Segura E, García J M, Campos A
Department of Cell Biology, Faculty of Medicine, University of Granada, Spain.
Histol Histopathol. 1990 Jul;5(3):305-10.
The interaction between human natural killer (NK) cells and NK-susceptible target cells, as well as the mechanism involved in target cell lysis, were studied with scanning electron microscopy (SEM). Low density human peripheral blood lymphocytes, highly enriched with large granular lymphocytes (LGL), were used as effector cells, and K562-cells were used as NK-susceptible target cells. The surface features of LGL/NK cells were examined under SEM. In the area of interaction, NK/target-cell conjugates showed microvilli and/or filipodia, and extensive areas of intercellular contact. In addition, the effector cells in some NK/target-cell conjugates were polarized toward the target cell. Changes in target cell surface features included loss of microvilli, large surface blebs and the appearance of small pore-like lesions on the cell membrane. Our findings show that target cell lysis occurred by apoptosis and plasma membrane lesions analogous to those seen during complement-mediated cytotoxicity.
利用扫描电子显微镜（SEM）研究了人类自然杀伤（NK）细胞与NK敏感靶细胞之间的相互作用以及靶细胞裂解所涉及的机制。以富含大颗粒淋巴细胞（LGL）的低密度人外周血淋巴细胞作为效应细胞，以K562细胞作为NK敏感靶细胞。在扫描电子显微镜下检查了LGL/NK细胞的表面特征。在相互作用区域，NK/靶细胞结合物显示出微绒毛和/或丝状伪足，以及广泛的细胞间接触区域。此外，一些NK/靶细胞结合物中的效应细胞向靶细胞极化。靶细胞表面特征的变化包括微绒毛丧失、大的表面泡以及细胞膜上出现小孔样病变。我们的研究结果表明，靶细胞裂解是通过凋亡和类似于补体介导的细胞毒性中所见的质膜损伤而发生的。
