基于 Ru(bpy)3(2+)-包封脂质体作为标记物的电化学发光免疫传感器用于检测甲胎蛋白
Electrochemiluminescence immunosensor for α-fetoprotein using Ru(bpy)3(2+)-encapsulated liposome as labels.
机构信息
Anhui Key Laboratory of Chemo-biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
出版信息
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):515-9. doi: 10.1016/j.colsurfb.2011.02.008. Epub 2011 Feb 26.
In this work, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of α-fetoprotein (AFP) was fabricated using Ru(bpy)(3)(2+)-encapsulated liposome as the label and electrodeposited gold nanoparticles (GNPs) as the immobilizing support. Great signal amplification was achieved since liposome could encapsulate large amount of reporter molecules and GNPs could provide large active surface. Under optimized conditions, with sandwich type format, a linear range of AFP from 0.005 to 0.2 pg/mL and an extremely low detection limit of 0.001 pg/mL was obtained, much lower than that in previous reports. The proposed ECL immnuosensor showed high sensitivity, specificity, and good stability, which may open a new door to ultrasensitive detection of proteins in clinical analysis.
在这项工作中，我们制备了一种基于电致化学发光（ECL）的免疫传感器，用于超灵敏检测甲胎蛋白（AFP）。该传感器使用包裹 Ru(bpy)(3)(2+)的脂质体作为标记物，电沉积金纳米粒子（GNPs）作为固定化支撑。由于脂质体可以包裹大量的报告分子，GNPs 可以提供大量的活性表面，因此实现了巨大的信号放大。在优化条件下，采用三明治型结构，我们获得了 AFP 的线性检测范围为 0.005 至 0.2 pg/mL，检测限低至 0.001 pg/mL，远低于之前的报道。所提出的 ECL 免疫传感器具有高灵敏度、特异性和良好的稳定性，为临床分析中蛋白质的超灵敏检测开辟了新途径。
Zotero 插件