利用时间相关单光子计数技术进行华法林的原位检测。

In situ detection of warfarin using time-correlated single-photon counting.

机构信息

Bioorganic and Biophysical Chemistry Laboratory, School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden.

出版信息

Biochem Biophys Res Commun. 2011 Apr 1;407(1):60-2. doi: 10.1016/j.bbrc.2011.02.103. Epub 2011 Feb 24.

DOI:10.1016/j.bbrc.2011.02.103

PMID:21354105

Abstract

Here we report on a novel method for the direct in situ measurement of specific isomeric forms of the anticoagulant warfarin using time correlated single-photon counting (TCSPC) spectroscopy in conjunction with synthetic Sudlow I binding site receptors. The method is highly robust over the clinically significant concentration range, and demonstrates the potential of the binding site mimics in conjunction with the spectroscopic strategy employed here for the determination of this important pharmaceutical in clinical or even environmental samples.

摘要

在这里，我们报告了一种使用时间相关单光子计数（TCSPC）光谱法结合合成 Sudlow I 结合位点受体直接原位测量抗凝剂华法林的特定异构体形式的新方法。该方法在临床显著浓度范围内具有高度的稳健性，并展示了结合位点模拟物与这里采用的光谱策略相结合在临床甚至环境样品中测定这种重要药物的潜力。