[使用分子技术鉴定临床相关真菌]
[Identification of clinically relevant fungi using molecular techniques].
作者信息
Unda Fabián, Agüero Jesús, Fariñas M Carmen, Martínez-Martínez Luis
机构信息
Servicio de Microbiología Clínica y Parasitología, Hospital Universitario Marqués de Valdecilla, Santander, España.
出版信息
Enferm Infecc Microbiol Clin. 2011 Apr;29(4):282-5. doi: 10.1016/j.eimc.2010.12.011. Epub 2011 Feb 26.
OBJECTIVE
To apply a sequencing-based molecular method to identify clinically relevant fungi to species level.
METHOD
Thirty-six fungi not identifiable at a species level by a conventional approach and 39 invasive clinical samples were prospectively evaluated. The results were compared with those obtained by conventional methods.
RESULTS
Molecular methods allowed rapid and reliable identification of fungi at species level, including both from organisms grown in culture and those in clinical samples.
CONCLUSION
Molecular methods show clear advantages for fungal identification, including rapid identification at species level and high negative predictive value.
目的
应用基于测序的分子方法将临床相关真菌鉴定到种水平。
方法
前瞻性评估36株用传统方法无法鉴定到种水平的真菌和39份侵袭性临床样本。将结果与传统方法获得的结果进行比较。
结果
分子方法能够快速、可靠地将真菌鉴定到种水平，包括培养物中的真菌和临床样本中的真菌。
结论
分子方法在真菌鉴定方面显示出明显优势，包括种水平的快速鉴定和高阴性预测值。
Zotero 插件