Sun Wan-sen, Zhao Yan-long, Ren Yan-yun, Ma Qiao-ya
Department of Traditional Chinese Medicine, Xi an Jiaotong University College of Medicine, Xi an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Feb;31(2):244-7.
To observe the effects of Qufengtongluo Recipe (QFTLR) on the expression of podocin mRNA and podocyte morphology in rats with adriamycin-induced nephropathy (AN), and explore the possible mechanism mediating the therapeutic effect of QFTLR on nephropathic proteinuria.
SD rats were randomized into normal control group, AN model group (established by a single injection of adriamycin via the tail vein), and 3 intervention groups with QFTLR, prednisone, or benazepril treatment. After the corresponding treatments, the expression of podocin mRNA in the renal tissues was detected by RT-PCR methods, and the morphological changes of the podocytes were examined by electron microscope.
Compared with the normal control group, the AN model group showed significantly lowered expressions of podocin mRNA (P<0.01) with reduced podocytes and widening, fusion or even absence of the foot processes (FP). Intervention with QFTLR significantly increased the expression of podocin mRNA (P<0.01) and the number of podocytes, and obviously lessened the structural changes of the FP.
QFTLR can produce therapeutic effect against nephropathic proteinuria possibly by up-regulating the expression of podocin mRNA and improving the morphological changes of the podocytes.
观察祛风通络方（QFTLR）对阿霉素诱导的肾病（AN）大鼠足突蛋白（podocin）mRNA表达及足细胞形态的影响，探讨QFTLR治疗肾病性蛋白尿的可能作用机制。
将SD大鼠随机分为正常对照组、AN模型组（通过尾静脉单次注射阿霉素建立）及3个干预组，分别给予QFTLR、泼尼松或苯那普利治疗。相应处理后，采用RT-PCR法检测肾组织中podocin mRNA的表达，电镜观察足细胞形态变化。
与正常对照组比较，AN模型组podocin mRNA表达明显降低（P<0.01），足细胞数量减少，足突增宽、融合甚至消失。QFTLR干预后，podocin mRNA表达明显增加（P<0.01），足细胞数量增多，足突结构改变明显减轻。
QFTLR可能通过上调podocin mRNA表达、改善足细胞形态变化而发挥治疗肾病性蛋白尿的作用。
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