Chazotte Brad
CSH Protoc. 2008 Feb 1;2008:pdb.prot4931. doi: 10.1101/pdb.prot4931.
INTRODUCTIONThe eukaryotic cell has evolved to compartmentalize its functions and transport various metabolites among cellular compartments. Therefore, in cell biology, the study of organization and structure/function relationships are of great importance. The Golgi apparatus is composed of a series of flattened, disk-shaped cisternae typically located near the cell nucleus. It is thought that the Golgi may be a principal organizer of macromolecular traffic in the cell because many molecules, such as secreted proteins, glycoproteins, glycolipids, and plasma membrane glycoproteins, pass through the Golgi during their maturation. In this protocol, fluorescent probes of the Golgi make use of this function to allow labeling of the Golgi.
引言
真核细胞已经进化出将其功能进行区室化并在细胞区室之间运输各种代谢物的能力。因此，在细胞生物学中，对组织以及结构/功能关系的研究非常重要。高尔基体由一系列通常位于细胞核附近的扁平盘状潴泡组成。人们认为高尔基体可能是细胞中大分子运输的主要组织者，因为许多分子，如分泌蛋白、糖蛋白、糖脂和质膜糖蛋白，在成熟过程中都会经过高尔基体。在本实验方案中，高尔基体的荧光探针利用这一功能来实现对高尔基体的标记。
