非洲爪蟾的外胚层（动物帽）层分离

Ectodermal (Animal Cap) Layer Separations in Xenopus laevis.

作者信息

Sive Hazel L, Grainger Robert M, Harland Richard M

出版信息

CSH Protoc. 2007 Jun 1;2007:pdb.prot4746. doi: 10.1101/pdb.prot4746.

Abstract

INTRODUCTIONIn Xenopus laevis, the blastula animal cap comprises two morphologically distinct cell layers, an outer monolayer termed the epithelial layer, which consists of tightly adherent pigmented cells, and an inner layer several cells thick termed the sensorial layer, which consists of loosely adherent cells. It is possible to isolate cell layers and to test their developmental potential and response to induction. This protocol describes how to separate the cell layers most easily, that is, by dissecting an intact Xenopus embryo.

摘要

引言

在非洲爪蟾中，囊胚动物帽由两个形态上不同的细胞层组成，一个外层单层称为上皮层，由紧密粘附的色素细胞组成，另一个内层有几层细胞厚，称为感觉层，由松散粘附的细胞组成。分离细胞层并测试它们的发育潜能和对诱导的反应是可能的。本方案描述了如何最容易地分离细胞层，即通过解剖完整的非洲爪蟾胚胎。