• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CalO1的结构表征：一种在刺孢霉素生物合成途径中假定的苔色酸甲基转移酶。

Structural characterization of CalO1: a putative orsellinic acid methyltransferase in the calicheamicin-biosynthetic pathway.

作者信息

Chang Aram, Singh Shanteri, Bingman Craig A, Thorson Jon S, Phillips George N

机构信息

Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

出版信息

Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):197-203. doi: 10.1107/S090744491100360X. Epub 2011 Feb 15.

DOI:10.1107/S090744491100360X
PMID:21358050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3046457/
Abstract

The X-ray structure determination at 2.4 Å resolution of the putative orsellinic acid C3 O-methyltransferase (CalO1) involved in calicheamicin biosynthesis is reported. Comparison of CalO1 with a homology model of the functionally related calicheamicin orsellinic acid C2 O-methyltransferase (CalO6) implicates several residues that are likely to contribute to the regiospecificity of alkylation. Consistent with the proposed requirement of an acyl-carrier-protein-bound substrate, this structural study also reveals structural determinants within CalO1 that are anticipated to accommodate an association with an acyl carrier protein.

摘要

报道了参与刺孢霉素生物合成的假定苔色酸C3 O-甲基转移酶（CalO1）在2.4 Å分辨率下的X射线结构测定。将CalO1与功能相关的刺孢霉素苔色酸C2 O-甲基转移酶（CalO6）的同源模型进行比较，发现了几个可能有助于烷基化区域特异性的残基。与酰基载体蛋白结合底物的拟议要求一致，这项结构研究还揭示了CalO1内的结构决定因素，预计这些因素可适应与酰基载体蛋白的结合。

相似文献

1
Structural characterization of CalO1: a putative orsellinic acid methyltransferase in the calicheamicin-biosynthetic pathway.CalO1的结构表征：一种在刺孢霉素生物合成途径中假定的苔色酸甲基转移酶。
Acta Crystallogr D Biol Crystallogr. 2011 Mar;67(Pt 3):197-203. doi: 10.1107/S090744491100360X. Epub 2011 Feb 15.
2
Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: a case of challenging structure determination at low resolution.来自加利车霉素生物合成途径的O-甲基转移酶CalO6的晶体结构：一个低分辨率下具有挑战性的结构测定案例。
BMC Struct Biol. 2015 Jul 15;15:13. doi: 10.1186/s12900-015-0040-6.
3
Characterization of the calicheamicin orsellinate C2-O-methyltransferase CalO6.加利车霉素苔色酸酯C2-O-甲基转移酶CalO6的特性分析
Chembiochem. 2014 Jul 7;15(10):1418-21. doi: 10.1002/cbic.201402119.
4
Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis.参与刺孢霉素生物合成的鼠李糖3'-O-甲基转移酶CalS11的结构与功能表征
ACS Chem Biol. 2013 Jul 19;8(7):1632-9. doi: 10.1021/cb400068k. Epub 2013 May 23.
5
Structural characterization of CalO2: a putative orsellinic acid P450 oxidase in the calicheamicin biosynthetic pathway.CalO2的结构表征：一种在加利车霉素生物合成途径中假定的苔色酸P450氧化酶。
Proteins. 2009 Jan;74(1):50-60. doi: 10.1002/prot.22131.
6
The Structure of the Bifunctional Everninomicin Biosynthetic Enzyme EvdMO1 Suggests Independent Activity of the Fused Methyltransferase-Oxidase Domains.双功能埃维诺霉素生物合成酶EvdMO1的结构表明融合的甲基转移酶-氧化酶结构域具有独立活性。
Biochemistry. 2018 Dec 18;57(50):6827-6837. doi: 10.1021/acs.biochem.8b00836. Epub 2018 Dec 7.
7
Structural Basis of the Selectivity of GenN, an Aminoglycoside N-Methyltransferase Involved in Gentamicin Biosynthesis.庆大霉素生物合成中氨基糖苷N - 甲基转移酶GenN选择性的结构基础
ACS Chem Biol. 2017 Nov 17;12(11):2779-2787. doi: 10.1021/acschembio.7b00466. Epub 2017 Oct 9.
8
Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.刺孢霉素生物合成早期糖基化步骤的生化与结构解析
Chem Biol. 2008 Aug 25;15(8):842-53. doi: 10.1016/j.chembiol.2008.06.011.
9
Identification and characterization of a methionine γ-lyase in the calicheamicin biosynthetic cluster of Micromonospora echinospora.棘孢小单孢菌中加利车霉素生物合成簇中一种甲硫氨酸γ-裂合酶的鉴定与表征
Chembiochem. 2015 Jan 2;16(1):100-9. doi: 10.1002/cbic.201402489. Epub 2014 Nov 17.
10
Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose.探究参与 D-四酮糖生物合成的 C-3'-甲基转移酶的催化机制。
Protein Sci. 2012 Jun;21(6):876-86. doi: 10.1002/pro.2074. Epub 2012 Apr 24.

引用本文的文献

1
Anticancer Effect of Mycotoxins From : Exploration of Natural Product Potential.来自……的霉菌毒素的抗癌作用：天然产物潜力的探索
Int J Microbiol. 2024 Dec 5;2024:5553860. doi: 10.1155/ijm/5553860. eCollection 2024.
2
Investigation of the Biosynthetic Mechanism of Bipentaromycin Featuring an Unprecedented Cyclic Head-to-Tail Dimeric Scaffold.具有前所未有的环状头对头二聚体支架的双戊霉素生物合成机制研究。
JACS Au. 2022 Dec 30;3(1):195-203. doi: 10.1021/jacsau.2c00594. eCollection 2023 Jan 23.
3
Crystal structures of PigF, an -methyltransferase involved in the prodigiosin synthetic pathway, reveal an induced-fit substrate-recognition mechanism.灵菌红素合成途径中一种α-甲基转移酶PigF的晶体结构揭示了一种诱导契合的底物识别机制。
IUCrJ. 2022 Mar 1;9(Pt 2):316-327. doi: 10.1107/S2052252521011696.
4
Rational Reprogramming of O-Methylation Regioselectivity for Combinatorial Biosynthetic Tailoring of Benzenediol Lactone Scaffolds.理性调控 O-甲基化区域选择性，实现苯二酚内酯骨架的组合生物合成后修饰。
J Am Chem Soc. 2019 Mar 13;141(10):4355-4364. doi: 10.1021/jacs.8b12967. Epub 2019 Feb 27.
5
The Structure of the Bifunctional Everninomicin Biosynthetic Enzyme EvdMO1 Suggests Independent Activity of the Fused Methyltransferase-Oxidase Domains.双功能埃维诺霉素生物合成酶EvdMO1的结构表明融合的甲基转移酶-氧化酶结构域具有独立活性。
Biochemistry. 2018 Dec 18;57(50):6827-6837. doi: 10.1021/acs.biochem.8b00836. Epub 2018 Dec 7.
6
Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: a case of challenging structure determination at low resolution.来自加利车霉素生物合成途径的O-甲基转移酶CalO6的晶体结构：一个低分辨率下具有挑战性的结构测定案例。
BMC Struct Biol. 2015 Jul 15;15:13. doi: 10.1186/s12900-015-0040-6.
7
Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2.地霉素糖基转移酶LanGT2的O-糖基化和C-糖基化变体的结构表征
Angew Chem Int Ed Engl. 2015 Feb 23;54(9):2811-5. doi: 10.1002/anie.201409792. Epub 2015 Jan 7.
8
Characterization of the calicheamicin orsellinate C2-O-methyltransferase CalO6.加利车霉素苔色酸酯C2-O-甲基转移酶CalO6的特性分析
Chembiochem. 2014 Jul 7;15(10):1418-21. doi: 10.1002/cbic.201402119.

本文引用的文献

1
[27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.[27] 用于多同晶置换和多波长反常衍射方法的最大似然重原子参数精修
Methods Enzymol. 1997;276:472-494. doi: 10.1016/S0076-6879(97)76073-7.
2
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
3
Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes.聚酮合酶化学并不能指导 9-和 10-元烯二炔生物合成的差异。
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11331-5. doi: 10.1073/pnas.1003442107. Epub 2010 Jun 7.
4
MolProbity: all-atom structure validation for macromolecular crystallography.MolProbity：用于大分子晶体学的全原子结构验证
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. doi: 10.1107/S0907444909042073. Epub 2009 Dec 21.
5
Searching protein structure databases with DaliLite v.3.使用DaliLite v.3搜索蛋白质结构数据库。
Bioinformatics. 2008 Dec 1;24(23):2780-1. doi: 10.1093/bioinformatics/btn507. Epub 2008 Sep 25.
6
Structural characterization of CalO2: a putative orsellinic acid P450 oxidase in the calicheamicin biosynthetic pathway.CalO2的结构表征：一种在加利车霉素生物合成途径中假定的苔色酸P450氧化酶。
Proteins. 2009 Jan;74(1):50-60. doi: 10.1002/prot.22131.
7
Comparative protein structure modeling using MODELLER.使用MODELLER进行比较蛋白质结构建模。
Curr Protoc Protein Sci. 2007 Nov;Chapter 2:Unit 2.9. doi: 10.1002/0471140864.ps0209s50.
8
The biosynthetic genes encoding for the production of the dynemicin enediyne core in Micromonospora chersina ATCC53710.编码嗜热小单孢菌ATCC53710中达尼霉素烯二炔核心生物合成的基因。
FEMS Microbiol Lett. 2008 May;282(1):105-14. doi: 10.1111/j.1574-6968.2008.01112.x. Epub 2008 Mar 5.
9
A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis.一种产生线性多烯以启动烯二炔类抗肿瘤抗生素生物合成的磷酸泛酰巯基乙胺化聚酮合酶。
Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1460-5. doi: 10.1073/pnas.0711625105. Epub 2008 Jan 25.
10
A short history of SHELX.SHELX简史。
Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22. doi: 10.1107/S0108767307043930. Epub 2007 Dec 21.