Piroozmand Ahmad, Hassan Zuhair M
Department of Microbiology and Immunology, Kashan University of Medical Sciences, Kashan, Iran.
J Cancer Res Ther. 2010 Oct-Dec;6(4):478-81. doi: 10.4103/0973-1482.77110.
To evaluate natural killer (NK) cells activity in breast cancer patients and its comparison with normal individuals.
Breast cancer is the most prevalent type of spontaneous tumor in humans, and NK cells are the first line defense against such tumors. There is a reverse correlation between NK activity and metastasis and reducing the tumor mass by surgery may be monitoring the NK activity. In this study, we evaluate NK activity in pre and post mastectomy patients.
Eighteen patients with invasive ductal carcinoma attended to cancer research institute were included in this study. NK cells were evaluated in pre and post mastectomy patients. PBMCs were isolated by ficoll hypaque. NK cell activity (% lysis of K562) was evaluated by flow cytometer.
One way analysis of variation (ANOVA) and Kruskal Wallis nonparametric test were employed using SPSS software.
While NK cell activity was greatly impaired in breast cancer patients (average lysis of K562 target cells: 24.4% vs. 62.5% in healthy controls, n = 18), it was found to be significantly increased after mastectomy (37.7%).
Mastectomy may lead to increased activity of NK cells among patients suffering from breast cancer and their increased activity may produce positive therapeutic effect.
评估乳腺癌患者的自然杀伤（NK）细胞活性，并将其与正常个体进行比较。
乳腺癌是人类最常见的自发性肿瘤类型，而NK细胞是抵御此类肿瘤的第一道防线。NK活性与转移之间存在负相关，手术切除肿瘤可能会监测NK活性。在本研究中，我们评估了乳房切除术前和术后患者的NK活性。
本研究纳入了18例就诊于癌症研究所的浸润性导管癌患者。对乳房切除术前和术后的患者进行NK细胞评估。通过Ficoll淋巴细胞分离液分离外周血单个核细胞（PBMC）。采用流式细胞仪评估NK细胞活性（K562细胞的裂解率）。
使用SPSS软件进行单因素方差分析（ANOVA）和Kruskal Wallis非参数检验。
虽然乳腺癌患者的NK细胞活性严重受损（K562靶细胞的平均裂解率：24.4%，而健康对照组为62.5%，n = 18），但发现乳房切除术后NK细胞活性显著增加（37.7%）。
乳房切除术可能会导致乳腺癌患者NK细胞活性增加，其活性增加可能会产生积极的治疗效果。
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