Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, United States.
ACS Nano. 2011 Mar 22;5(3):1606-16. doi: 10.1021/nn1025719. Epub 2011 Feb 25.
A common challenge in nanotechnology is the fabrication of materials with well-defined nanoscale structure and properties. Here we report that a genetically engineered tobacco mosaic virus (TMV) coat protein (CP), to which a hexahistidine (His) tag was incorporated, can self-assemble into disks, hexagonally packed arrays of disks, stacked disks, helical rods, fibers, and elongated rafts. The insertion of a His tag to the C-terminus of TMV-CP was shown to significantly affect the self-assembly in comparison to the wild type, WT-TMV-CP. Furthermore, the His tag interactions attributed to the alternative self-assembly of His-TMV-CP can be controlled through ethanol and nickel-nitrilotriacetic acid (Ni-NTA) additions as monitored with atomic force microscopy.
在纳米技术中，一个常见的挑战是制造具有良好定义的纳米级结构和性能的材料。在这里，我们报告说，一种经过基因工程改造的烟草花叶病毒（TMV）外壳蛋白（CP），其中掺入了六组氨酸（His）标签，可以自组装成盘状、盘状六边形排列、堆叠盘状、螺旋状杆、纤维和伸长的筏子。与野生型 TM V-CP 相比，向 TMV-CP 的 C 末端插入 His 标签显着影响自组装。此外，通过原子力显微镜监测到，His 标签相互作用归因于 His-TMV-CP 的替代自组装，可以通过添加乙醇和镍-亚氨基二乙酸（Ni-NTA）来控制。
