建立 CE-ESI-microTOF-MS 方法，用于快速鉴定荞麦中的酚类化合物。

Development of a CE-ESI-microTOF-MS method for a rapid identification of phenolic compounds in buckwheat.

机构信息

Dipartimento di Scienze degli Alimenti, Università di Bologna, Cesena (FC) Italy.

出版信息

Electrophoresis. 2011 Mar;32(6-7):669-73. doi: 10.1002/elps.201000609. Epub 2011 Mar 1.

DOI:10.1002/elps.201000609

Abstract

A sensitive CE-ESI-MS analytical method for the identification of buckwheat antioxidants has been developed. CE and ESI-TOF parameters (e.g. buffer composition and pH, sheath liquid composition, sheath liquid and gas flow rates, electrospray voltage) were optimized to obtain an optimal analytical separation and identification. The results confirmed the presence of phenolic acids, procyanidins and galloylated propelargonidins. The identification of swertiamacroside and 2-hydroxy-3-O-β-D-glucopyranosil-benzoic acid, found for the first time in our previous work, has been confirmed. Furthermore, 5,7,4'-trimethoxyflavan and dihydroxy-trimethoxyisoflavan have also been tentatively identified for the first time in buckwheat.

摘要

已经开发出一种用于鉴定荞麦抗氧化剂的灵敏 CE-ESI-MS 分析方法。优化了 CE 和 ESI-TOF 参数（例如缓冲液组成和 pH 值、鞘液组成、鞘液和气体流速、电喷雾电压），以获得最佳的分析分离和鉴定。结果证实了存在酚酸、原花青素和没食子酰基丙氨酸。首次在我们之前的工作中发现了 swertiamacroside 和 2-羟基-3-O-β-D-吡喃葡萄糖基苯甲酸的存在，并得到了确认。此外，荞麦中还首次暂定鉴定出 5,7,4'-三甲氧基黄酮和二羟基三甲氧基异黄酮。