Department of Emergency Surgery, the Red Cross Hospital in Hangzhou, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang Province 310003, PR China.
Ann Diagn Pathol. 2011 Jun;15(3):162-9. doi: 10.1016/j.anndiagpath.2010.10.004. Epub 2011 Mar 2.
Glypican 3 (GPC3) is a glycosylphosphatidylinositol-anchored membrane protein and plays an important role in regulation of cell growth, differentiation, and migration. The aims of this study were to investigate the expression of GPC3 in human liver, biliary tract, and pancreatic tumors and to evaluate its diagnostic role in differentiating hepatocellular carcinoma (HCC) from other hepatic mimickers. Immunohistochemistry was performed on a large collection of surgically resected samples from 941 primary liver tumors, 50 metastatic adenocarcinomas, and 30 normal livers as well as primary adenocarcinomas of the pancreas (n = 17), gallbladder (n = 30), and extrahepatic bile duct (n = 20). The relationship of GPC3 expression and clinicopathologic features in patients with HCC was determined. We found that 516 (52%) of the 991 liver neoplastic tissue samples demonstrated positive staining for GPC3. A high incidence of GPC3 expression (492/757; 65%) was observed in HCC, whereas intrahepatic cholangiocarcinomas, adenocarcinomas, and benign liver lesions displayed rare positive cases. There were significant correlations between GPC3 expression and clinicopathologic characteristics, including histologic grade (P < .001), intrahepatic metastasis (P = .007), and positive serum hepatitis B surface antigen (P = .042), in patients with HCC. In conclusion, our results confirm the high expression of GPC3 in HCC and suggest its potential diagnostic value as a clinical marker for this disease.
Glypican 3（GPC3）是一种糖基磷脂酰肌醇锚定的膜蛋白，在调节细胞生长、分化和迁移方面发挥着重要作用。本研究旨在探讨 GPC3 在人肝、胆道和胰腺肿瘤中的表达，并评估其在鉴别肝细胞癌（HCC）与其他肝类似物中的诊断作用。我们对 991 例原发性肝癌、50 例转移性腺癌和 30 例正常肝以及 17 例胰腺原发性腺癌、30 例胆囊和 20 例肝外胆管原发性腺癌的大量手术切除样本进行了免疫组织化学染色。确定了 GPC3 表达与 HCC 患者临床病理特征的关系。我们发现，991 例肝肿瘤组织样本中有 516 例（52%）对 GPC3 呈阳性染色。在 HCC 中观察到 GPC3 表达的高发生率（492/757；65%），而肝内胆管癌、腺癌和良性肝病变则显示出罕见的阳性病例。GPC3 表达与包括组织学分级（P<0.001）、肝内转移（P=0.007）和乙型肝炎表面抗原阳性血清（P=0.042）在内的临床病理特征之间存在显著相关性。总之，我们的结果证实了 GPC3 在 HCC 中的高表达，并提示其作为该疾病临床标志物的潜在诊断价值。
